Antibody Specificity Profiling Using Protein Microarrays.
Antibody Specificity Profiling Using Protein Microarrays.
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使用蛋白质微阵列进行抗体特异性分析。
DOI：
10.1007/978-1-4939-7841-0_14
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Blackshaw,Seth
中科院分区：
文献类型：
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作者：
Ramos-López,Pedro;Irizarry,José;Pino,Ignacio;Blackshaw,Seth
抗体是用于分离和检测特定蛋白质的最广泛使用的试剂。然而，在这些研究中使用特异性不高的抗体可能会产生不准确和误导性的数据。蛋白质微阵列提供了一个平台，通过该平台可以同时定量分析抗体针对多种细胞抗原的交叉反应性。该方案详细描述了阵列预处理、抗体结合、洗涤、扫描和抗体特异性定量分析的过程。
Antibodies are the most widely used reagent for isolation and detection of specific proteins. However, using antibodies that are not highly specific in these studies can generate inaccurate and misleading data. Protein microarrays offer a platform by which antibody cross-reactivity against a broad range of cellular antigens can be simultaneously and quantitatively profiled. This protocol describes in detail the process of array pretreatment, antibody binding, washing, scanning and quantitative analysis of antibody specificity.