Microfluidic Assisted Nanoprecipitation of PLGA Nanoparticles for Curcumin Delivery to Leukemia Jurkat Cells
Microfluidic Assisted Nanoprecipitation of PLGA Nanoparticles for Curcumin Delivery to Leukemia Jurkat Cells
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DOI：
10.1021/acs.langmuir.7b04335
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发表时间：
2018-04-03
期刊：
影响因子：
3.9
通讯作者：
Shen, Amy Q.
中科院分区：
文献类型：
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作者：
Leung, Mandy H. M.;Shen, Amy Q.
控制用于药物递送的纳米颗粒的粒度和粒度分布的能力是至关重要的，因为它影响纳米颗粒的生物分布和细胞摄取。我们提出了一种新的微流控辅助纳米沉淀策略，该策略能够合成无表面活性剂的姜黄素封装的聚（丙交酯-共-乙交酯）纳米颗粒（Cur-PLGA NP），其具有可调节的粒径（30-70 nm）和窄的粒径分布（多分散指数小于0.2）。我们的Cur-PLGA NP具有出色的胶体稳定性并抑制姜黄素的降解。我们进一步证明了我们的Cur-PLGA NP作为姜黄素的纳米毒性递送系统的潜力。细胞活力测定验证了Cur-PLGA NP在白血病Jurkat细胞中的剂量依赖性细胞毒性。相反，Cur-PLGA NP不改变成纤维细胞NIH 3 T3细胞的活力，这表明Cur-PLGA NP的细胞毒性对细胞类型是特异性的。此外，PLGA NP对白血病Jurkat细胞和成纤维细胞NIH 3 T3细胞都没有可检测的作用，突出了我们的递送系统的无毒性质。共聚焦细胞摄取研究表明PLGA NP不改变姜黄素的细胞摄取。我们的微流体辅助方法为姜黄素提供了一种可控和有效的纳米生物材料合成药物递送系统，其可以扩展到各种生物医学应用的不同胶囊材料。
The ability to control particle size and size distribution of nanoparticles for drug delivery is essential because it impacts on the biodistribution and cellular uptake of nanoparticles. We present a novel microfluidic assisted nanoprecipitation strategy that enables synthesis of surfactant-free curcumin encapsulated poly(lactide-co-glycolide) nanoparticles (Cur-PLGA NP) with adjustable particle diameters (30-70 nm) and narrow particle size distribution (polydispersity index less than 0.2). Our Cur-PLGA NP exhibit excellent colloidal stability and inhibit degradation of curcumin. We further demonstrate the potential of our Cur-PLGA NP as a nanotoxic delivery system for curcumin. Cellular viability assay validates a dose-dependent cytotoxicity of Cur-PLGA NP in leukemia Jurkat cells. In contrast, Cur-PLGA NP does not alter the viability of fibroblast NIH3T3 cells, which suggests that the cytotoxicity of Cur-PLGA NP is specific to cell types. Furthermore, there is no detectable effect by PLGA NP to both leukemia Jurkat cells and fibroblast NIH3T3 cells, highlighting the nontoxic nature of our delivery system. Confocal cell uptake studies indicate that PLGA NP do not alter the cell uptake of curcumin. Our microfluidic assisted approach offers a controlled and effective nanobiomaterials synthesis of drug delivery system for curcumin, which can be extended to different capsule materials for a variety of biomedical applications.