Identification of antigenic proteins from salivary glands of female Anopheles maculatus by proteomic analysis
Identification of antigenic proteins from salivary glands of female Anopheles maculatus by proteomic analysis
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DOI：
10.1016/j.apjtb.2016.08.012
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发表时间：
2016-11-01
影响因子：
1.7
通讯作者：
Sardjono, Teguh Wahju
中科院分区：
文献类型：
--
作者：
Armiyanti, Yunita;Arifianto, Renam Putra;Sardjono, Teguh Wahju
目的：用蛋白质组学方法鉴定雌性斑点按蚊唾液腺抗原蛋白，为血清学工具寻找候选生物标志物。方法：采用一维凝胶电泳法(十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法)、免疫印迹和液-质联用技术对斑点按蚊唾液腺抗原蛋白进行鉴定。结果：分子量分别为43和34 kDa的蛋白为抗原蛋白。Mascot Server的计算生物信息学分析揭示了7个新的假设蛋白质(MW：43 kDa)和两个新的假设蛋白质(MW：34 kDa)。进一步的分析(BLASTP、抗原性、表位定位和特异性分析)表明，在达林按蚊中鉴定出两个新的蛋白质，分别为载脂蛋白D和组织蛋白酶D。结论：所鉴定的蛋白质有望成为蚊虫叮咬暴露的生物标志物。
Objective: To identify antigenic proteins from the salivary glands of female Anopheles maculatus using a proteomic approach to find the biomarker candidate for serological tools.Methods: The identification of antigenic proteins of Anopheles maculatus salivary gland used these techniques: one-dimensional gel electrophoresis (sodium dodecyl sulfate polyacrylamide gel electrophoresis), western blot, and liquid chromatography-mass spectrometry.Results: The proteins that have molecular weight (MW) 43 and 34 kDa were the antigenic protein. Computational bioinformatic analysis by Mascot Server revealed seven novel hypothetical proteins (MW: 43 kDa) and two novel hypothetical proteins (MW: 34 kDa). Further analysis (BLASTP, antigenicity, epitope mapping, and specificity analysis) showed that two novel proteins were identified as apolipoprotein D and cathepsin D in Anopheles darlingi.Conclusions: The identified proteins are potential to be developed as a biomarker of mosquito bite's exposure.