Single-Cell Transcriptomics Reveals the Cellular Heterogeneity of Cardiovascular Diseases.
Single-Cell Transcriptomics Reveals the Cellular Heterogeneity of Cardiovascular Diseases.
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DOI：
10.3389/fcvm.2021.643519
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发表时间：
2021
影响因子：
3.6
通讯作者：
Song J
中科院分区：
文献类型：
--
作者：
Fu M;Song J
“一朵野花里有一个世界，一片叶子里有一个菩提”，小小的细胞里蕴藏着巨大的秘密。血管系统由许多多功能细胞亚群组成，每个细胞亚群都与心血管疾病的发生和发展有关。单细胞转录组学捕捉单个细胞内表达的基因的全貌，识别罕见或从头开始的细胞亚群，分析单细胞轨迹和干细胞或祖细胞谱系转换，并在单细胞分辨率下比较健康组织和疾病相关组织。单细胞转录组学在过去的十年中对心血管研究领域产生了深远的影响，这体现在心血管细胞图谱的构建，以及对心血管疾病和干细胞或祖细胞分化机制的阐明。血管系统中细胞亚群的分类和比例因物种、位置、基因和疾病的不同而不同，在器官发育、疾病进展和退化方面表现出独特的基因表达特征。特定的基因标记物有望成为疾病的诊断标准、治疗靶点或预后指标。因此，血管疾病的治疗仍有很大的发展潜力。在此，我们对正常血管和动脉粥样硬化、主动脉瘤和肺动脉高压中的细胞团和基因表达模式进行综述，以揭示单细胞转录组技术在心血管疾病中的血管异质性和新的调控因素，并讨论其目前的局限性和潜在的临床应用前景。
“A world in a wild flower, and a bodhi in a leaf,” small cells contain huge secrets. The vasculature is composed of many multifunctional cell subpopulations, each of which is involved in the occurrence and development of cardiovascular diseases. Single-cell transcriptomics captures the full picture of genes expressed within individual cells, identifies rare or de novo cell subpopulations, analyzes single-cell trajectory and stem cell or progenitor cell lineage conversion, and compares healthy tissue and disease-related tissue at single-cell resolution. Single-cell transcriptomics has had a profound effect on the field of cardiovascular research over the past decade, as evidenced by the construction of cardiovascular cell landscape, as well as the clarification of cardiovascular diseases and the mechanism of stem cell or progenitor cell differentiation. The classification and proportion of cell subpopulations in vasculature vary with species, location, genotype, and disease, exhibiting unique gene expression characteristics in organ development, disease progression, and regression. Specific gene markers are expected to be the diagnostic criteria, therapeutic targets, or prognostic indicators of diseases. Therefore, treatment of vascular disease still has lots of potentials to develop. Herein, we summarize the cell clusters and gene expression patterns in normal vasculature and atherosclerosis, aortic aneurysm, and pulmonary hypertension to reveal vascular heterogeneity and new regulatory factors of cardiovascular disease in the use of single-cell transcriptomics and discuss its current limitations and promising clinical potential.