Mechanical compression and hydrostatic pressure induce reversible changes in actin cytoskeletal organisation in chondrocytes in agarose
Mechanical compression and hydrostatic pressure induce reversible changes in actin cytoskeletal organisation in chondrocytes in agarose
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DOI：
10.1016/j.jbiomech.2005.04.006
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发表时间：
2006-01-01
影响因子：
2.4
通讯作者：
Bader, DL
中科院分区：
文献类型：
--
作者：
Knight, MM;Toyoda, T;Bader, DL
在许多细胞类型中，细胞骨架已被广泛地牵涉到涉及拉伸激活离子通道、整合素和细胞内细胞器变形的机械转导途径。研究还表明，细胞骨架可以响应于诸如拉伸应变或流体流动的机械刺激而进行重塑。在关节软骨细胞中，机械传导途径是复杂的，相互关联的，但迄今为止，知之甚少。此外，很少有人知道软骨细胞骨架如何响应生理机械负荷。本研究利用良好的特征软骨细胞-琼脂糖模型和一个既定的共聚焦图像分析技术，以证明静态和循环，压缩应变和静水压力都诱导肌动蛋白微丝重塑。这种重塑的特点是从一个统一的变化，以更点状分布的皮质肌动蛋白细胞周围。对于某些加载制度，这种重塑逆转了随后的I小时卸载期间。因此，这种可逆的肌动蛋白细胞骨架重塑可能代表了一种机制，通过这种机制，软骨细胞改变其机械性能和机械敏感性，以响应生理机械负荷。(c)2005爱思唯尔有限公司保留所有权利。
In numerous cell types, the cytoskeleton has been widely implicated in mechanotransduction pathways involving stretch-activated ion channels, integrins and deformation of intracellular organelles. Studies have also demonstrated that the cytoskeleton can undergo remodelling in response to mechanical stimuli such as tensile strain or fluid flow. In articular chondrocytes, the mechanotransduction pathways are complex, inter-related and as yet, poorly understood. Furthermore, little is known of how the chondrocyte cytoskeleton responds to physiological mechanical loading. This study utilises the well-characterised chondrocyte-agarose model and an established confocal image-analysis technique to demonstrate that both static and cyclic, compressive strain and hydrostatic pressure all induce remodelling of actin microfilaments. This remodelling was characterised by a change from a uniform to a more punctate distribution of cortical actin around the cell periphery. For some loading regimes, this remodelling was reversed over a subsequent I h unloaded period. This reversible remodelling of actin cytoskeleton may therefore represent a mechanism through which the chondrocyte alters its mechanical properties and mechanosensitivity in response to physiological mechanical loading. (c) 2005 Elsevier Ltd. All rights reserved.