DIRECT GENE-TRANSFER INTO MOUSE MUSCLE INVIVO
DIRECT GENE-TRANSFER INTO MOUSE MUSCLE INVIVO
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DOI：
10.1126/science.1690918
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发表时间：
1990-03-23
期刊：
影响因子：
56.9
通讯作者：
FELGNER, PL
中科院分区：
文献类型：
--
作者：
WOLFF, JA;MALONE, RW;FELGNER, PL
含有氯霉素乙酰转移酶、荧光素酶和。β基因的RNA和DNA表达载体。-半乳糖苷酶分别注射到小鼠骨骼肌中。在所有情况下，蛋白表达都很容易被检测到，并且不需要特殊的递送系统来实现这些效果。RNA和DNA结构的表达程度与体外最佳条件下转染成纤维细胞的表达程度相当。原位细胞化学染色。-半乳糖苷酶活性定位于肌肉细胞。-半乳糖苷酶DNA载体。注射DNA荧光素酶表达载体后，荧光素酶活性在肌肉中至少存在2个月。
RNA and DNA expression vectors containing genes for chloramphenicol acetyltransferase, luciferase, and .beta.-galactosidase were separately injected into mouse skeletal muscle in vivo. Protein expression was readily detected in all cases, and no special delivery system was required for these effects. The extent of expression from both the RNA and DNA constructs was comparable to that obtained from fibroblasts transfected in vitro under optimal conditions. In situ cytochemical staining for .beta.-galactosidase activity was localized to muscle cells following injection of the .beta.-galactosidase DNA vector. After injection of the DNA luciferase expression vector, luciferase activity was present in the muscle for at least 2 months.