An acyl-SAM analog as an affinity ligand for identifying quorum sensing signal synthases
An acyl-SAM analog as an affinity ligand for identifying quorum sensing signal synthases
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DOI：
10.1039/c4cc03094j
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发表时间：
2014-08-11
影响因子：
4.9
通讯作者：
Hayashi, Hideo
中科院分区：
文献类型：
--
作者：
Kai, Kenji;Fujii, Hiroki;Hayashi, Hideo
N-酰基高丝氨酸内酯(AHLS)是由革兰氏阴性菌产生的群体感应信号。本文报道了以催化中间体酰基-SAM为基础设计的酶抑制剂偶联小球亲和纯化AHL合成酶的方法。
N-Acylhomoserine lactones (AHLs) are quorum sensing signals produced by Gram-negative bacteria. We here report the affinity purification of AHL synthases using beads conjugated with an enzyme inhibitor, which was designed based on the catalytic intermediate acyl-SAM.