Changes in the Soil Microbiome in Eggplant Monoculture Revealed by High-Throughput Illumina MiSeq Sequencing as Influenced by Raw Garlic Stalk Amendment
Changes in the Soil Microbiome in Eggplant Monoculture Revealed by High-Throughput Illumina MiSeq Sequencing as Influenced by Raw Garlic Stalk Amendment
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DOI：
10.3390/ijms20092125
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发表时间：
2019-05-01
影响因子：
5.6
通讯作者：
Cheng, Zhihui
中科院分区：
文献类型：
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作者：
Ghani, Muhammad Imran;Ali, Ahmad;Cheng, Zhihui
植物残体在土壤中的掺入可以被认为是改善土壤结构功能的关键可持续性因素。然而，植物残体的添加对土壤微生物群落的生化循环和非生物胁迫现象的影响知之甚少。通过研究生蒜秸秆（RGS）对土壤化学性质和微生物群落结构的影响，探讨生蒜秸秆（RGS）改良剂在避免单作相关生产限制方面的作用。RGS以四种不同剂量施用，即对照（RGS0）、1%（RGS1）、3%（RGS2）和5%（RGS3）/100 g土壤。RGS改良剂显著提高了土壤电导率（EC），N，P，K和酶活性。施用RGS显著降低了土壤pH值。高通量Illumina MiSeq测序揭示了响应于RGS应用的细菌群落结构的显著改变。在检测到的23个主要类群中，Anaerolineaceae，Acidobacteria和Cyanobacteria的丰度水平增加。RGS2增加了一些据报道有益的细菌，包括酸杆菌、芽孢杆菌和浮游菌（分别增加了42%、64%和1%）。此外，真菌的内转录间隔区（ITS）在不同的处理之间显示出显著的多样性，分类群如毛壳菌属（56.2%），枝顶孢属（4.3%），镰刀菌属（4%），曲霉属（3.4%），Sordariomycetes（3%），和Plectosphaerellaceae（2%）显示出丰富的。有趣的是，鸡腿菇（14%），观察到只有在RGS修正的土壤。RGS处理有效地改变了土壤真菌群落结构，并减少了某些已知的致病真菌属，即，镰刀菌属和枝顶孢属。本研究的结果表明，RGS修正案可能会影响微生物群落结构，可能会影响茄子的生理和形态属性下的塑料大棚蔬菜栽培系统（PGVC）在单作。
The incorporation of plant residues into soil can be considered a keystone sustainability factor in improving soil structure function. However, the effects of plant residue addition on the soil microbial communities involved in biochemical cycles and abiotic stress phenomena are poorly understood. In this study, experiments were conducted to evaluate the role of raw garlic stalk (RGS) amendment in avoiding monoculture-related production constraints by studying the changes in soil chemical properties and microbial community structures. RGS was applied in four different doses, namely the control (RGS0), 1% (RGS1), 3% (RGS2), and 5% (RGS3) per 100 g of soil. The RGS amendment significantly increased soil electrical conductivity (EC), N, P, K, and enzyme activity. The soil pH significantly decreased with RGS application. High-throughput Illumina MiSeq sequencing revealed significant alterations in bacterial community structures in response to RGS application. Among the 23 major taxa detected, Anaerolineaceae, Acidobacteria, and Cyanobacteria exhibited an increased abundance level. RGS2 increased some bacteria reported to be beneficial including Acidobacteria, Bacillus, and Planctomyces (by 42%, 64%, and 1% respectively). Furthermore, internal transcribed spacer (ITS) fungal regions revealed significant diversity among the different treatments, with taxa such as Chaetomium (56.2%), Acremonium (4.3%), Fusarium (4%), Aspergillus (3.4%), Sordariomycetes (3%), and Plectosphaerellaceae (2%) showing much abundance. Interestingly, Coprinellus (14%) was observed only in RGS-amended soil. RGS treatments effectively altered soil fungal community structures and reduced certain known pathogenic fungal genera, i.e., Fusarium and Acremonium. The results of the present study suggest that RGS amendment potentially affects the microbial community structures that probably affect the physiological and morphological attributes of eggplant under a plastic greenhouse vegetable cultivation system (PGVC) in monoculture.