Structural basis for anticodon recognition by methionyl-tRNA synthetase
Structural basis for anticodon recognition by methionyl-tRNA synthetase
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DOI：
10.1038/nsmb988
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发表时间：
2005-10-01
影响因子：
16.8
通讯作者：
Nureki, O
中科院分区：
文献类型：
--
作者：
Nakanishi, K;Ogiso, Y;Nureki, O
在与tRNA（Met）和甲硫基腺苷酸类似物配合物的2.7埃分辨率晶体结构中，tRNA反密码子环扭曲形成由C34、A35和A38组成的三碱基堆叠。一个色氨酸残基叠加在C34上扩展了三碱基叠加。C34与Arg357形成watson - crick型氢键。这种结构解决了长期存在的MetRS如何特异性识别tRNA（Met）的问题。
In the 2.7-angstrom resolution crystal structure of methionyl-tRNA synthetase (MetRS) in complex with tRNA(Met) and a methionyladenylate analog, the tRNA anticodon loop is distorted to form a triple-base stack comprising C34, A35 and A38. A tryptophan residue stacks on C34 to extend the triple-base stack. In addition, C34 forms Watson-Crick-type hydrogen bonds with Arg357. This structure resolves the longstanding question of how MetRS specifically recognizes tRNA(Met).