The dynamic response of the Arabidopsis root metabolome to auxin and ethylene is not predicted by changes in the transcriptome
The dynamic response of the Arabidopsis root metabolome to auxin and ethylene is not predicted by changes in the transcriptome
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DOI：
10.1038/s41598-019-57161-9
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发表时间：
2020-01-20
影响因子：
4.6
通讯作者：
Winkel, Brenda S. J.
中科院分区：
文献类型：
--
作者：
Hildreth, Sherry B.;Foley, Evan E.;Winkel, Brenda S. J.
虽然植物激素对植物基因表达的影响已得到很好的表征，但对激素如何影响代谢产物谱知之甚少。本研究采用高分辨率的24小时时间过程，与时间匹配的转录组学分析平行进行，研究了生长素和乙烯升高对拟南芥根代谢组学的影响。采用正离子和负离子模式下的正交UPLC分离策略（反相和HILIC）进行质谱分析，以最大限度地鉴别水平改变的代谢物。研究结果表明，根代谢组对激素刺激反应迅速，属于同一类代谢物的化合物表现出类似的变化。的反应占主导地位的苯丙素，硫代葡萄糖苷，和脂肪酸代谢的变化，虽然响应的性质和时间是独特的每种激素。代谢组中的这些改变不能通过相应的转录组数据直接预测，这表明转录后事件如酶活性和/或转运过程的变化驱动了代谢组中观察到的变化。这些研究结果强调，需要更好地了解植物代谢的时间重构的生化机制，特别是在有关的代谢组界面及其随后的生理和形态学影响。
While the effects of phytohormones on plant gene expression have been well characterized, comparatively little is known about how hormones influence metabolite profiles. This study examined the effects of elevated auxin and ethylene on the metabolome of Arabidopsis roots using a high-resolution 24h time course, conducted in parallel to time-matched transcriptomic analyses. Mass spectrometry using orthogonal UPLC separation strategies (reversed phase and HILIC) in both positive and negative ionization modes was used to maximize identification of metabolites with altered levels. The findings show that the root metabolome responds rapidly to hormone stimulus and that compounds belonging to the same class of metabolites exhibit similar changes. The responses were dominated by changes in phenylpropanoid, glucosinolate, and fatty acid metabolism, although the nature and timing of the response was unique for each hormone. These alterations in the metabolome were not directly predicted by the corresponding transcriptome data, suggesting that post-transcriptional events such as changes in enzyme activity and/or transport processes drove the observed changes in the metabolome. These findings underscore the need to better understand the biochemical mechanisms underlying the temporal reconfiguration of plant metabolism, especially in relation to the hormone-metabolome interface and its subsequent physiological and morphological effects.