A transcriptomics resource for wheat functional genomics
A transcriptomics resource for wheat functional genomics
复制标题
DOI：
10.1111/j.1467-7652.2004.00096.x
复制
发表时间：
2004-11-01
影响因子：
13.8
通讯作者：
Edwards, KJ
中科院分区：
文献类型：
--
作者：
Wilson, ID;Barker, GLA;Edwards, KJ
在非生物胁迫下的谷物发育、发芽和植物发育是谷物群落非常感兴趣的生物学领域。在研究基因功能项目中，我们已经生产了研究六倍体小麦在这些发育过程中转录组变化所需的资源。我们从35个cDNA文库中随机挑选了700到1300个克隆，这些克隆代表了谷物和植物发育的高度特定阶段。带注释的测序结果已存储在可公开访问的在线数据库http://www.cerealsdb.uk.net中。所使用的每个组织阶段也都被详细拍摄了下来，从而形成了一组高质量的显微图像，详细描述了小麦籽粒的发育。这些图片经过整理和注释，以便制作一个以小麦发展为重点的网站（http://www.wheatbp.net/）。我们还基于35个cDNA文库制作了一个公开可用的小麦单基因集的高密度微阵列，并完成了一些微阵列实验，以验证其质量。
Grain development, germination and plant development under abiotic stresses are areas of biology that are of considerable interest to the cereal community. Within the Investigating Gene Function programme we have produced the resources required to investigate alterations in the transcriptome of hexaploid wheat during these developmental processes. We have single pass sequenced the cDNAs of between 700 and 1300 randomly picked clones from each of 35 cDNA libraries representing highly specific stages of grain and plant development. Annotated sequencing results have been stored in a publicly accessible, online database at http://www.cerealsdb.uk.net. Each of the tissue stages used has also been photographed in detail, resulting in a collection of high-quality micrograph images detailing wheat grain development. These images have been collated and annotated in order to produce a web site focused on wheat development (http://www.wheatbp.net/). We have also produced high-density microarrays of a publicly available wheat unigene set based on the 35 cDNA libraries and have completed a number of microarray experiments which validate their quality.