The role of serum response factor in early coronary vasculogenesis.
The role of serum response factor in early coronary vasculogenesis.
复制标题
血清反应因子在早期冠状血管发生中的作用。
DOI：
10.1007/s00246-009-9614-z
复制
发表时间：
2010
影响因子：
1.6
通讯作者：
Misra,RaviP
中科院分区：
文献类型：
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作者：
Misra,RaviP
血管发育缺陷是胎儿死亡和先天性心血管疾病的主要原因。知识是稀缺的胚胎血管祖细胞和潜在的调控机制，决定他们的承诺，各种血管细胞谱系。在心血管发育的早期，心外膜（PE）是血管祖细胞的主要来源。PE是在鸡的第16和17阶段、小鼠的第9.5天（dpc）和人类的约5周时发现的短暂胚胎结构，由覆盖横隔（ST）的间皮细胞和间充质细胞组成。PE内的细胞经历复杂的发育和分化过程，其涉及至少两个定向的迁移波和上皮向间充质转化（EMT）。PE细胞与发育中心肌的下表面接触，在形成心外膜的心脏表面上迁移，并渗透到心肌中并产生心外膜下间充质细胞（SEMC）。SEMC随后在整个发育的心脏中迁移，产生由成纤维细胞以及内皮细胞和平滑肌细胞组成的整个冠状血管系统[32]血清反应因子（SRF）对于胚胎发育期间的中胚层形成是必需的[2]。在禽类外植体模型中已经证明了SRF在由尚未表征的PE衍生的冠状血管前体细胞发育血管平滑肌中的必要作用[15]。SRF在哺乳动物PE介导的血管发育的一些早期步骤中的体内作用-
Defects in vascular development are a major cause of fetal demise and congenital cardiovascular disease. Knowledge is scarce concerning both embryonic vascular cell progenitors and the underlying regulatory mechanisms that determine their commitment to various vessel cell lineages. During early cardiovascular development, the proepicardium (PE) is the major source of vascular progenitors. The PE is a transient embryonic structure found at stages 16 and 17 in the chick, at 9.5 days postcoitum (dpc) in the mouse, and at approximately 5 weeks in the human that consists of mesothelial and mesenchymal cells overlying the septum transversum (ST). Cells within the PE undergo a complex process of development and differentiation that involves at least two directed waves of migration and epithelial-to-mesenchymal transitions (EMT). The PE cells make contact with the inferior surface of the developing myocardium, migrate over the surface of the heart forming the epicardium, and penetrate into the myocardium and give rise to subepicardial mesenchymal cells (SEMCs). The SEMCs subsequently migrate throughout the developing heart giving rise to the entire coronary vasculature consisting of fibroblasts as well as endothelial and smooth muscle cells [32].Serum response factor (SRF) is essential for mesoderm formation during embryonic development [2]. A necessary role for SRF in the development of vascular smooth muscle from yet to be characterized PE-derived coronary vascular precursor cells has been demonstrated in avian explant models [15]. The role of SRF in vivo during some of the earliest steps in mammalian PE-mediated vascular develop-