Deconstructing Pancreas Developmental Biology
Deconstructing Pancreas Developmental Biology
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DOI：
10.1101/cshperspect.a012401
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发表时间：
2012-06-01
影响因子：
7.2
通讯作者：
Kim, Seung K.
中科院分区：
文献类型：
--
作者：
Benitez, Cecil M.;Goodyer, William R.;Kim, Seung K.
人类胰腺疾病（特别是糖尿病和腺癌）的不断发展和患病率的不断增加，促使人们进一步了解胰腺器官发生。胰腺是一个多功能器官，其上皮细胞控制着多种重要的生理内分泌和外分泌功能。近年来，控制胰腺内分泌和外分泌成分的产生、分化、形态发生、生长、成熟和维持的机制被发现的速度越来越快。这包括最近的研究揭示了未成熟和成熟胰腺细胞亚群的发育潜力具有意想不到的灵活性的机制，包括相互转换命运的能力。在本文中，我们描述了如何通过强大的现代方法（包括表观遗传分析、延时成像和基于流式细胞术的细胞纯化）来补充经典细胞生物学、遗传分析、谱系追踪和胚胎学研究，以剖析胰腺发育的基本过程。
The relentless nature and increasing prevalence of human pancreatic diseases, in particular, diabetes mellitus and adenocarcinoma, has motivated further understanding of pancreas organogenesis. The pancreas is a multifunctional organ whose epithelial cells govern a diversity of physiologically vital endocrine and exocrine functions. The mechanisms governing the birth, differentiation, morphogenesis, growth, maturation, and maintenance of the endocrine and exocrine components in the pancreas have been discovered recently with increasing tempo. This includes recent studies unveiling mechanisms permitting unexpected flexibility in the developmental potential of immature and mature pancreatic cell subsets, including the ability to interconvert fates. In this article, we describe how classical cell biology, genetic analysis, lineage tracing, and embryological investigations are being complemented by powerful modern methods including epigenetic analysis, time-lapse imaging, and flow cytometry-based cell purification to dissect fundamental processes of pancreas development.