Reversal of a Single Base-Pair Step Controls Guanine Photo-Oxidation by an Intercalating Ruthenium(II) Dipyridophenazine Complex.
Reversal of a Single Base-Pair Step Controls Guanine Photo-Oxidation by an Intercalating Ruthenium(II) Dipyridophenazine Complex.
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DOI：
10.1002/anie.201502608
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发表时间：
2015-07-13
期刊：
影响因子：
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通讯作者：
Kelly JM
中科院分区：
文献类型：
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作者：
Keane PM;Poynton FE;Hall JP;Sazanovich IV;Towrie M;Gunnlaugsson T;Quinn SJ;Cardin CJ;Kelly JM
DNA序列的微小变化往往会产生重大的生物学效应。本文利用皮/纳秒瞬态可见光和时间分辨红外（TRIR）光谱，比较了Λ‐[Ru(TAP)2(dppz)]2+在5′‐{CCGGATCCGG}2和5′‐{CCGGTACCGG}2中光氧化鸟嘌素的速率和产率。电子转移在TA序列中的低效率与X射线晶体学预测的5 ‘ - TA - 3 ’与5 ‘ - AT - 3 ’结合偏好是一致的。TRIR光谱还揭示了两种寡核苷酸结合位点的差异。
Small changes in DNA sequence can often have major biological effects. Here the rates and yields of guanine photo‐oxidation by Λ‐[Ru(TAP)2(dppz)]2+ have been compared in 5′‐{CCGGATCCGG}2 and 5′‐{CCGGTACCGG}2 using pico/nanosecond transient visible and time‐resolved IR (TRIR) spectroscopy. The inefficiency of electron transfer in the TA sequence is consistent with the 5′‐TA‐3′ versus 5′‐AT‐3′ binding preference predicted by X‐ray crystallography. The TRIR spectra also reveal the differences in binding sites in the two oligonucleotides.