Surface interleukin-10 inhibits listericidal activity by primary macrophages.
Surface interleukin-10 inhibits listericidal activity by primary macrophages.
复制标题
表面白细胞介素 10 抑制初级巨噬细胞的杀李斯特菌活性。
DOI：
10.1002/jlb.66.6.961
复制
发表时间：
1999
影响因子：
5.5
通讯作者：
Campbell,PA
中科院分区：
文献类型：
--
作者：
Fleming,SD;Leenen,PJ;Freed,JH;Campbell,PA
Interleukin‐10 (IL‐10) down‐regulates multiple functions of monocytes and macrophages, including the ability of macrophages to kill many intracellular microorganisms. The experiments presented here test the hypothesis that IL‐10 expressed on the cell surface inhibits the ability of primary mouse macrophages to kill the facultative, intracellular bacteriumListeria monocytogenes.We show that, in contrast to macrophages from normal mice, both bone marrow‐derived macrophages (BMDM) and thioglycollate‐elicited macrophages obtained from IL‐10−/−mice can killL. monocytogenes.Treatment with anti‐IL‐10 monoclonal antibody (mAb) enables BMDM from normal mice and thioglycollate‐elicited macrophages from RAG‐2−/−mice (which lack T or B cell‐derived IL‐10) to killL. monocytogenes, and concurrently down‐regulates the expression of surface IL‐10. Surface IL‐10 on paraformaldehyde‐fixed cells can inhibit nitric oxide (NO) production by interferon‐γ (IFN‐γ)‐stimulated macrophages from IL‐10−/−mice, thus directly showing functional activity of surface IL‐10. Taken together, these studies indicate that macrophage surface IL‐10 is biologically active and down‐regulates macrophage bactericidal activity.J. Leukoc. Biol.66: 961–967; 1999.
