Mutation rate analysis via parent-progeny sequencing of the perennial peach. I. A low rate in woody perennials and a higher mutagenicity in hybrids.
Mutation rate analysis via parent-progeny sequencing of the perennial peach. I. A low rate in woody perennials and a higher mutagenicity in hybrids.
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通过多年生桃子的亲子测序进行突变率分析。
DOI：
10.1098/rspb.2016.1016
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发表时间：
2016-10-26
期刊：
影响因子：
--
通讯作者：
Hurst LD
中科院分区：
文献类型：
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作者：
Xie Z;Wang L;Wang L;Wang Z;Lu Z;Tian D;Yang S;Hurst LD
突变率在物种之间、物种内的菌株之间以及基因组内的区域之间变化。这些变异形式的决定因素是什么？在这里，通过对桃的亲子测序，我们询问（i）与一年生植物相比，木本多年生植物是否倾向于具有较低的单位时间突变率，以及（ii）杂交菌株是否具有较高的突变率。在来自种内杂交的低杂合性个体的叶片与其自交后代的叶片之间，突变率为每代7.77 × 10−9个点突变/bp，与拟南芥（每代7.0-7.4 × 10−9个点突变/bp）相似。这表明单位时间突变率较低，因为桃的世代时间要长得多。我们估计从200年历史的低杂合性桃子到其后代每一代每bp有9.48 × 10 - 9个点突变，这支持了这一点。从种间杂交产生的高度杂合的个体到其自交后代，突变率为每代每个位点1.38 × 10−8个突变，与杂交后代的突变率提高一致。因此，我们的数据表明，（一）桃有大约一个数量级的单位时间比拟南芥低的突变率，与木本多年生植物的低进化率的报告一致，和（二）杂交可能，确实，与增加突变率，考虑在世纪前。
Mutation rates vary between species, between strains within species and between regions within a genome. What are the determinants of these forms of variation? Here, via parent–offspring sequencing of the peach we ask whether (i) woody perennials tend to have lower per unit time mutation rates compared to annuals, and (ii) hybrid strains have high mutation rates. Between a leaf from a low heterozygosity individual, derived from an intraspecific cross, to a leaf of its selfed progeny, the mutation rate is 7.77 × 10−9 point mutations per bp per generation, similar to Arabidopsis thaliana (7.0–7.4 × 10−9 point mutations per bp per generation). This suggests a low per unit time mutation rate as the generation time is much longer in peach. This is supported by our estimate of 9.48 × 10−9 point mutations per bp per generation from a 200-year-old low heterozygosity peach to its progeny. From a more highly heterozygous individual derived from an interspecific cross to its selfed progeny, the mutation rate is 1.38 × 10−8 mutations per site per generation, consistent with raised rates in hybrids. Our data thus suggest that (i) peach has an approximately order of magnitude lower mutation rate per unit time than Arabidopsis, consistent with reports of low evolutionary rates in woody perennials, and (ii) hybridization may, indeed, be associated with increased mutation rates as considered over a century ago.