The utility of automated analysis of inter-simple sequence repeat (ISSR) loci for resolving relationships in the Canary Island species of Tolpis (Asteraceae)
The utility of automated analysis of inter-simple sequence repeat (ISSR) loci for resolving relationships in the Canary Island species of Tolpis (Asteraceae)
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DOI：
10.3732/ajb.93.8.1154
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发表时间：
2006-08-01
影响因子：
3
通讯作者：
Mort, Mark E.
中科院分区：
文献类型：
--
作者：
Archibald, Jenny K.;Crawford, Daniel J.;Mort, Mark E.
海洋岛屿上的植物在形态上往往与大陆上的近亲非常不同，它们是研究进化和物种形成的有用模型，因为进化在时间和空间上是重叠的。先前的研究表明，在约10个加那利岛的托尔皮斯物种分支中，DNA序列变异很小，这排除了解决物种关系的可能性。本研究使用来自36个已知物种群体的264个个体和三个未描述的形态变异，评估了自动分析ISSR基因座在解决支系内部关系方面的作用。相似度(Dice系数)和Fitch简约度分别用于生成邻居加入树(NJ)和严格共识树(MP)。形态上不同的特有种的所有个体在两棵树上都形成了簇。在NJ树中还支持两个未描述的变体的集群。主要由两个物种组成的形态可变复合体的个体在种群或物种水平上没有得到很好的分辨。新泽西州和MP树在更深层次上是不一致的，包括物种之间的关系。结果从物种生物学的角度进行了解释，并讨论了ISSR标记自动分析在解释加那利群岛托尔皮斯进化模式中的作用。
Plants of oceanic islands, often remarkably divergent morphologically from continental relatives, are useful models for studying evolution and speciation because evolution is telescoped in time and space. Prior studies revealed little DNA sequence variation within the clade of ca. 10 Canary Island species of Tolpis, which precluded resolving species relationships. The present study assessed the utility of automated analysis of inter-simple sequence repeat (ISSR) loci for resolving relationships within the clade using 264 individuals from 36 populations of all recognized species and three undescribed morphological variants. Similarity (Dice coefficient) and Fitch parsimony were used to generate neighbor-joining (NJ) and strict consensus trees (MP), respectively. All individuals of the morphologically distinct endemic species formed clusters in both trees. There is also support for clusters of two undescribed variants in the NJ tree. Individuals from a morphologically variable complex consisting primarily of two species are not well resolved at population or species levels. The NJ and MP trees are not congruent at deeper levels, including relationships among species. Results are interpreted in terms of the biology of the species, and the utility of automated analysis of ISSR markers for interpreting patterns of evolution of Tolpis in the Canary Islands is discussed.