Ventricular K+ currents are reduced in mice with elevated levels of serum TNFα
Ventricular K+ currents are reduced in mice with elevated levels of serum TNFα
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DOI：
10.1016/j.yjmcc.2009.02.025
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发表时间：
2009-08-01
影响因子：
5
通讯作者：
Fiset, Celine
中科院分区：
文献类型：
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作者：
Grandy, Scott A.;Fiset, Celine
在本研究中，小鼠接受肿瘤坏死因子α（TNF α）治疗6周，以确定长期TNF α治疗是否可产生与疾病状态（心力衰竭，HIV）中观察到的相似的TNF α血清水平，并确定这些TNF α水平是否改变心室K+电流。将用TNF α长期处理的小鼠和假处理的小鼠用于实验。用Searchlight（R）蛋白阵列测量血清水平。采用膜片钳技术、实时荧光定量PCR和Western印迹分析方法研究细胞膜上钾电流密度和钾通道表达。结果显示，TNF α治疗组小鼠的血清TNF α浓度显著高于对照组（对照组：9.5 +/- 1.5 pg/ml，TNF α：27.4 +/- 5.0 pg/ml; p
In the present study mice were treated with tumor necrosis factor alpha (TNF alpha) for 6 weeks to determine if chronic TNF alpha treatment could produce serum levels of TNF alpha similar to what has been observed in disease states (heart failure, HIV) and to determine if these levels of TNF alpha alter ventricular K+ currents. Mice chronically treated with TNF alpha and sham treated mice were utilized for experiments. Serum levels were measured with a Searchlight (R) protein array. Patch-clamp techniques, real-time PCR and Western blot analysis were used to study K+ current densities and K+ channel expression. Results showed that serum concentrations of TNF alpha were significantly higher in TNF alpha treated mice compared to controls (control: 9.5 +/- 1.5 pg/ml, TNF alpha: 27.4 +/- 5.0 pg/ml; p