Metabolomic Screening Applied to Rice FOX Arabidopsis Lines Leads to the Identification of a Gene-Changing Nitrogen Metabolism
Metabolomic Screening Applied to Rice FOX Arabidopsis Lines Leads to the Identification of a Gene-Changing Nitrogen Metabolism
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DOI：
10.1093/mp/ssp069
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发表时间：
2010-01-01
期刊：
影响因子：
27.5
通讯作者：
Saito, Kazuki
中科院分区：
文献类型：
--
作者：
Albinsky, Doris;Kusano, Miyako;Saito, Kazuki
植物代谢组学是研究基因功能和深入了解植物细胞生理的有力工具。在这项研究中，我们筛选过表达水稻全长（FL）cDNA的拟南芥品系（水稻FOX拟南芥品系）使用气相色谱-飞行时间质谱（GC-TOF/MS）为基础的技术，以确定水稻基因引起的代谢变化。该筛选系统允许快速和可靠地鉴定显示代谢物谱改变的候选品系。我们进行了代谢组学和转录组学分析的水稻FOX拟南芥线，窝藏FL cDNA的水稻直向同源物的侧向器官边界（LOB）域（LBD）/不对称Leoprotein 2-like（ASL）基因的拟南芥，At-LBD 37/ASL 39。所研究的水稻FOX拟南芥系在与氮代谢相关的代谢物水平上表现出显著的变化。转录组学数据以及拟南芥At-LBD 37/ASL 39-过表达植物的代谢物分析结果与这些发现一致。此外，对Os-LBD 37/ASL 39过表达水稻植株的代谢组学和转录组学分析表明，Os-LBD 37/ASL 39与水稻氮代谢相关的过程有关。因此，基于代谢组学的筛选方法和功能获得方法的组合对于快速表征拟南芥和水稻中的新基因是有用的。
Plant metabolomics developed as a powerful tool to examine gene functions and to gain deeper insight into the physiology of the plant cell. In this study, we screened Arabidopsis lines overexpressing rice full-length (FL) cDNAs (rice FOX Arabidopsis lines) using a gas chromatography-time-of-flight mass spectrometry (GC-TOF/MS)-based technique to identify rice genes that caused metabolic changes. This screening system allows fast and reliable identification of candidate lines showing altered metabolite profiles. We performed metabolomic and transcriptomic analysis of a rice FOX Arabidopsis line that harbored the FL cDNA of the rice ortholog of the Lateral Organ Boundaries (LOB) Domain (LBD)/Asymmetric Leaves2-like (ASL) gene of Arabidopsis, At-LBD37/ASL39. The investigated rice FOX Arabidopsis line showed prominent changes in the levels of metabolites related to nitrogen metabolism. The transcriptomic data as well as the results from the metabolite analysis of the Arabidopsis At-LBD37/ASL39-overexpressor plants were consistent with these findings. Furthermore, the metabolomic and transcriptomic analysis of the Os-LBD37/ASL39-overexpressing rice plants indicated that Os-LBD37/ASL39 is associated with processes related to nitrogen metabolism in rice. Thus, the combination of a metabolomics-based screening method and a gain-of-function approach is useful for rapid characterization of novel genes in both Arabidopsis and rice.