Calcium oxalate nephrolithiasis:: Effect of renal crystal deposition on the cellular composition of the renal interstitium
Calcium oxalate nephrolithiasis:: Effect of renal crystal deposition on the cellular composition of the renal interstitium
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DOI：
10.1016/s0272-6386(99)70231-3
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发表时间：
1999-04-01
影响因子：
13.2
通讯作者：
Schröder, FH
中科院分区：
文献类型：
--
作者：
de Water, R;Noordermeer, C;Schröder, FH
尿草酸钙（CaOx）晶体和晶体团块通常无害地排出，但在肾结石中，它们被肾小管上皮细胞保留并转移到肾间质中。这种晶体物质诱导由间质细胞数量增加和细胞外基质扩张组成的炎症反应。新到达的细胞要么来自血液或结缔组织，要么通过局部增殖形成，识别间质晶体周围的细胞是研究间质细胞是否可以去除晶体物质的第一步。因此，我们对由乙二醇（EG）和氯化铵（AC）引起的高尿酸大鼠的肾脏进行了免疫组织化学研究，注意到识别所有类型白细胞的白细胞共同抗原（LCA）、特异于单核细胞和巨噬细胞的EDI抗原以及存在于树突状细胞上的主要组织相容性II类抗原（MHC II）的表达，B淋巴细胞和活化的巨噬细胞。将所得结果与急性和慢性草酸盐中毒的两个人肾脏标本中所见的结果进行了比较。在大鼠和人中，巨噬细胞和多核巨细胞是包裹间质晶体的主要细胞。反应的这种相似性强调了大鼠肾结石模型的相关性。此外，大鼠实验表明，如果从饮用水中省略肾结石剂EG和AC，则间质晶体的数量和生化测量的肾相关草酸盐的量均随时间减少。进一步的研究必须阐明巨噬细胞和多核巨细胞是否能够清除间质晶体，以及这些细胞如何在炎症部位募集。(C)1999年由国家肾脏基金会，公司。
Urinary calcium oxalate (CaOx) crystals and crystal agglomerates are normally harmlessly excreted, but in nephrolithiasis they are retained by tubular epithelial cells and shifted into the renal interstitium, This crystalline material induces an inflammatory response consisting of an increase in the number of interstitial cells and an expansion of the extracellular matrix. The newly arrived cells either derive from the blood or the connective tissue or they are formed by local proliferation, Identification of the cells that surround the interstitial crystals is a first step in investigating the question of whether the interstitial cells could remove the crystalline material. Therefore, we performed an immunohistochemical study on the kidneys of rats made hyperoxaluric by ethylene glycol (EG) and ammonium chloride (AC), Attention was paid to expression of the leukocyte common antigen (LCA), which identifies all types of leukocytes, the EDI antigen, which is specific for monocytes and macrophages, and the major histocompatibility class II antigen (MHC II), which is present on dendritic cells, B lymphocytes, and activated macrophages. The results obtained were compared with those seen in two human kidney specimens with acute and chronic oxalosis, In both rat and humans, macrophages and multinucleated giant cells are the major cells that encapsulate the interstitial crystals. This similarity in response underlines the relevance of the rat nephrolithiasis model. The rat experiments showed, furthermore, that the number of interstitial crystals and the amount of biochemically measured kidney-associated oxalate both decrease with time, if the nephrolithiatic agents EG and AC are omitted from the drinking water. Further studies must clarify whether macrophages and multinucleated giant cells are able to remove the interstitial crystals and how these cells are recruited at the inflammatory site. (C) 1999 by the National Kidney Foundation, Inc.