Measuring glomerular number and size in perfused kidneys using MRI
Measuring glomerular number and size in perfused kidneys using MRI
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DOI：
10.1152/ajprenal.00044.2011
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发表时间：
2011-06-01
影响因子：
4.2
通讯作者：
Bennett, Kevin M.
中科院分区：
文献类型：
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作者：
Beeman, Scott C.;Zhang, Min;Bennett, Kevin M.
Beeman SC, Zhang M, Gubhaju L, Wu T, Bertram JF, Frakes DH, Cherry BR, Bennett KM。MRI测量灌注肾肾小球数量和大小。[J] .中国生物医学工程学报，2016,31(5):557 - 557。首次发表于2011年3月16日；doi: 10.1152 / ajprenal.00044.2011。这项工作的目的是无损地测量整个肾脏的肾小球（以及肾元）数量。肾小球数量和大小的变化与许多肾脏和全身性疾病有关。在这里，我们开发了一种基于注射阳离子铁蛋白（CF）的稳健磁共振成像（MRI）技术，以精确测量单个肾小球的数量和大小。体内静脉注射CF后灌注的大鼠肾脏的高场（19特斯拉）梯度回声MR图像显示CF遍布整个肾脏的单个肾小球的特异性标记。我们开发了一种三维图像处理算法来计数每个标记的肾小球。mri计数显示33,786 +/- 3,753个标记肾小球（n = 5个肾脏）。对侧肾脏酸浸计数估计有30,585 +/- 2,053个肾小球（n = 6个肾脏）。矢状/分块体视学计数估计有34,963个肾小球（n = 2）。基于mri的标记肾小球表观肾小球体积测量为4.89 x 10(-4) mm(3) (n = 5)，而平均立体测量为4.99 x 10(-4) mm(3) （n = 2）。基于mri的技术也产生了肾小球表观体积的肾内分布，这是以前在组织学上无法获得的测量。这项工作使得非破坏性地测量整个肾小球数量和表观肾小球体积来研究肾脏疾病的易感性成为可能，并为在动物和人类中进行类似的体内测量打开了大门。
Beeman SC, Zhang M, Gubhaju L, Wu T, Bertram JF, Frakes DH, Cherry BR, Bennett KM. Measuring glomerular number and size in perfused kidneys using MRI. Am J Physiol Renal Physiol 300: F1454-F1457, 2011. First published March 16, 2011; doi:10.1152/ajprenal.00044.2011.-The goal of this work was to nondestructively measure glomerular (and thereby nephron) number in the whole kidney. Variations in the number and size of glomeruli have been linked to many renal and systemic diseases. Here, we develop a robust magnetic resonance imaging (MRI) technique based on injection of cationic ferritin (CF) to produce an accurate measurement of number and size of individual glomeruli. High-field (19 Tesla) gradient-echo MR images of perfused rat kidneys after in vivo intravenous injection of CF showed specific labeling of individual glomeruli with CF throughout the kidney. We developed a three-dimensional image-processing algorithm to count every labeled glomerulus. MRI-based counts yielded 33,786 +/- 3,753 labeled glomeruli (n = 5 kidneys). Acid maceration counting of contralateral kidneys yielded an estimate of 30,585 +/- 2,053 glomeruli (n = 6 kidneys). Disector/fractionator stereology counting yielded an estimate of 34,963 glomeruli (n = 2). MRI-based measurement of apparent glomerular volume of labeled glomeruli was 4.89 x 10(-4) mm(3) (n = 5) compared with the average stereological measurement of 4.99 x 10(-4) mm(3) (n = 2). The MRI-based technique also yielded the intrarenal distribution of apparent glomerular volume, a measurement previously unobtainable in histology. This work makes it possible to nondestructively measure whole-kidney glomerular number and apparent glomerular volumes to study susceptibility to renal diseases and opens the door to similar in vivo measurements in animals and humans.