Photoreactivation of bacteriophages after UV disinfection: Role of genome structure and impacts of UV source
Photoreactivation of bacteriophages after UV disinfection: Role of genome structure and impacts of UV source
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DOI：
10.1016/j.watres.2014.01.065
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发表时间：
2014-05-15
期刊：
影响因子：
12.8
通讯作者：
Linden, Karl G.
中科院分区：
文献类型：
--
作者：
Rodriguez, Roberto A.;Bounty, Sarah;Linden, Karl G.
本文描述了MS2、phiX174、T1和PRD1噬菌体的紫外线灭活动力学，以及细菌紫外线修复机制重新激活这些噬菌体的可能性。选定的噬菌体代表了一系列基因组大小、单链和双链基因组、环状和线状组织以及RNA和DNA。用两种不同的准直光源(中压汞灯和低压汞灯)照射噬菌体，在此期间，将噬菌体培养物置于光复活光中6h，然后在37℃黑暗中孵育过夜。紫外线照射后的黑暗对照平行进行。UV灭活动力学(暗对照)表明，环状单链DNA噬菌体(PhiX174)是最敏感的，线形单链RNA噬菌体(MS2)是抗性最强的噬菌体。没有观察到MS2(RNA噬菌体)的光复活，而Prd1的光复活最高。在Prd1的情况下，4对数减少(暗对照)所需的剂量约为35mJ/cm(2)，两种紫外线源(MP和LP)观察到的剂量相似。当增加光复活步骤时，使用LP灯的4log还原所需的剂量为103mJ/cm(2)，使用MP灯的4log还原所需的剂量为60mJ/cm(2)。噬菌体之间的基因组结构差异在细菌宿主机制介导的抗紫外线灭活和潜在的光复活中起着重要作用。在检测PRD1的过程中使用光复活为UV挑战测试中的潜在使用创造了一个更保守的替代物。(C)2014爱思唯尔有限公司。保留所有权利。
The UV inactivation kinetics of bacteriophages MS2, PhiX174, T1 and PRD1 and the potential of bacterial UV repair mechanisms to reactivate these bacteriophages is described here. The selected bacteriophages represent a range of genome size, single and double stranded genomes, circular and linear organization and RNA and DNA. Bacteriophages were exposed to UV irradiation from two different collimated beam UV irradiation sources (medium-pressure (MP) mercury lamps and low-pressure (LP) mercury lamps) and assayed during which host-phage cultures were exposed to photoreactivating light for 6 h, then incubated overnight at 37 C in the dark. Dark controls following UV exposure were performed in parallel. UV inactivation kinetics (using dark controls) showed that circular ssDNA phage (PhiX174) was the most sensitive and linear ssRNA phage (MS2) was the more resistant phage. No photoreactivation was observed for MS2 (RNA phage) and the highest photoreactivation was observed for PRD1. In the case of PRD1, the dose required for 4-log reduction (dark control) was around 35 mJ/cm(2), with a similar dose observed for both UV sources (MP and LP). When the photoreactivation step was added, the dose required for 4-log reduction using LP lamps was 103 mj/cm(2) and for MP lamps was 60 mJ/cm(2). Genome organization differences between bacteriophages play an important role in resistance to UV inactivation and potential photoreactivation mediated by bacterial host mechanisms. The use of photoreactivation during the assay of PRD1 creates a more conservative surrogate for potential use in UV challenge testing. (C) 2014 Elsevier Ltd. All rights reserved.