TAXONOMY OF CLOSTRIDIA - WALL COMPOSITION AND DNA HOMOLOGIES IN CLOSTRIDIUM-BUTYRICUM AND OTHER BUTYRIC ACID-PRODUCING CLOSTRIDIA

TAXONOMY OF CLOSTRIDIA - WALL COMPOSITION AND DNA HOMOLOGIES IN CLOSTRIDIUM-BUTYRICUM AND OTHER BUTYRIC ACID-PRODUCING CLOSTRIDIA
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DOI:
10.1099/00221287-67-1-33
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发表时间:
1971-01-01
期刊:
JOURNAL OF GENERAL MICROBIOLOGY
影响因子:
--
通讯作者:
JOHNSON, JL
JOHNSON, JL
中科院分区:
其他
文献类型:
--
作者:
CUMMINS, CS;JOHNSON, JL

文献摘要

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根据壁糖模式、DNA制备的核苷酸序列相似性和营养需求,发现被命名为丁酸梭菌的菌株构成两个不同的类群。同源组1的细胞壁仅含葡萄糖,可在0.05 μg /ml的无机盐-葡萄糖培养基中生长。生物素,尽管通过添加氨基酸促进了生长。一些菌株标记为dc。multifermentansandC。法拉克斯属于这个群体。同源组II的生物以葡萄糖和半乳糖作为壁糖,除非添加酵母提取物,否则不能在含有氨基酸和10种维生素的无机盐-葡萄糖培养基中生长。离岸金融中心。溶淀粉菌、红孢梭菌、beijerinckic、乳嗜乙酰梭菌和一些标记为dc的菌株。多发菌属II类。建议将第一类生物命名为丁酸梭菌,而将第一类生物命名为丁酸梭菌。第二组建议使用beijerinckii。一些菌株标记为dc。和c的菌株。乙酰丁酸梭菌、金抗丁酸梭菌、巴氏梭菌等。tyrobutyricum不属于这两类。
Strains designated asClostridium butyricumwere found to constitute two distinct groups on the basis of wall sugar patterns, nucleotide-sequence similarities of DNA preparations and nutritional requirements. Organisms in homology group I had walls which contained only glucose and would grow in a mineral salts-glucose medium with 0·05 μg./ml. biotin, although growth was improved by the addition of amino acids. Some strains labelledC. multifermentansandC. fallaxbelonged to this group. Organisms in homology group II had glucose and galactose as wall sugars and would not grow in mineral salts-glucose medium with amino acids and 10 vitamins unless yeast extract was also added. Strains ofC. amylolyticum, C. rubrum, C. beijerinckii, C. lacto-acetophilumand some strains labelledC. multifermentansbelonged to group II.It is recommended that the nameClostridium butyricumbe retained for the organisms in group I, whileC. beijerinckiiis suggested for group II. Some of the strains labelledC. fallaxand the strains ofC. acetobutylicum, C. aurantibutyricum, C. pasteurianumandC. tyrobutyricumdid not belong to either group.