Chronic Wasting Disease (CWD) Susceptibility of Several North American Rodents That Are Sympatric with Cervid CWD Epidemics
Chronic Wasting Disease (CWD) Susceptibility of Several North American Rodents That Are Sympatric with Cervid CWD Epidemics
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DOI：
10.1128/jvi.00560-09
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发表时间：
2010-01-01
影响因子：
5.4
通讯作者：
Barr, Daniel J.
中科院分区：
文献类型：
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作者：
Heisey, Dennis M.;Mickelsen, Natalie A.;Barr, Daniel J.
慢性消耗性疾病（CWD）是一种高度传染性的致命性神经退行性疾病，目前已知仅自然感染鹿科（Cervidae）物种。在北美的几个地方，自由放养的鹿科动物正在发生慢性消耗病流行病，其他野生动物物种肯定也暴露在传染性物质中。为了评估传播的潜力，我们脑内接种四种CWD的同域啮齿动物。传播是有效的，在所有物种的发病速度更快的两个田鼠物种比两个Peromyscus属。从具有或不具有CWD抗性基因型的CWD阳性鹿制备的接种物的结果相似。第二次传代后存活时间显著缩短，表明适应性。与所有其他已知的在170位具有天冬酰胺的cricetid啮齿动物的朊病毒蛋白序列不同，我们的红背田鼠表达丝氨酸，并反驳了以前的建议，即在这个位置的丝氨酸大大降低了对慢性消耗病的易感性。鉴于这些啮齿动物物种的清除习惯，CWD朊病毒在环境中的明显持久性，以及这些啮齿动物不可避免地暴露于CWD朊病毒，我们的脑内挑战结果表明，有必要进一步调查自然传播的可能性。
Chronic wasting disease (CWD) is a highly contagious always fatal neurodegenerative disease that is currently known to naturally infect only species of the deer family, Cervidae. CWD epidemics are occurring in free-ranging cervids at several locations in North America, and other wildlife species are certainly being exposed to infectious material. To assess the potential for transmission, we intracerebrally inoculated four species of epidemic-sympatric rodents with CWD. Transmission was efficient in all species; the onset of disease was faster in the two vole species than the two Peromyscus spp. The results for inocula prepared from CWD-positive deer with or without CWD-resistant genotypes were similar. Survival times were substantially shortened upon second passage, demonstrating adaptation. Unlike all other known prion protein sequences for cricetid rodents that possess asparagine at position 170, our red-backed voles expressed serine and refute previous suggestions that a serine in this position substantially reduces susceptibility to CWD. Given the scavenging habits of these rodent species, the apparent persistence of CWD prions in the environment, and the inevitable exposure of these rodents to CWD prions, our intracerebral challenge results indicate that further investigation of the possibility of natural transmission is warranted.