The effect of thyroid hormone on the chromatin structure and expression of the malic enzyme gene in hepatocytes.
The effect of thyroid hormone on the chromatin structure and expression of the malic enzyme gene in hepatocytes.
复制标题
甲状腺激素对肝细胞染色质结构及苹果酸酶基因表达的影响。
DOI：
10.1210/mend-2-7-619
复制
发表时间：
1988
影响因子：
--
通讯作者：
V. Nikodem
中科院分区：
文献类型：
--
作者：
S. Usala;W. Young;H. Morioka;V. Nikodem
Malic enzyme catalyzes the NADP-dependent oxidative decarboxylation of malate to pyruvate and carbon dioxide and is involved in lipogenesis. We have investigated the effect of thyroid hormone on the chromatin structure of the malic enzyme gene in rat liver. Hypersensitivity to DNase I in the immediate 5'-flanking region was altered by T3. T3 stimulation induced hypersensitive sites at -310 base pairs (bp) and -50 bp whereas a hypersensitive site at -170 bp was thyroid hormone independent. Hypersensitive sites identified in the 3'-flanking region showed no change with T3 stimulation. We further characterized expression of the malic enzyme gene as a function of thyroidal state by localizing malic enzyme mRNA in hepatocytes using in situ hybridization histochemistry. In hypothyroid and euthyroid states, two populations of hepatocytes were seen, some with malic enzyme message and others with no detectable message. These differences in malic enzyme gene expression were most evident between groups or regions of hepatocytes. After 10 days of thyroid hormone treatment all hepatocytes demonstrated malic enzyme message. The hypersensitivity results confirm that thyroid hormone stimulation of malic enzyme synthesis occurs in part at the level of transcription, and localization of malic enzyme gene expression suggests this stimulation is accompanied by recruitment of hepatocytes. Hepatocytes may be heterogeneous in their ability to respond to thyroid hormone.
大鼠肝脏中甲状腺激素反应基因的染色质结构和甲基化状态。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jump,DB;Wong,NC;Oppenheimer,JH
通讯作者：
Oppenheimer,JH
雌激素诱导极低密度载脂蛋白 II（一种主要的禽肝卵黄蛋白）合成涉及肝细胞的募集。
影响因子：
4.8
作者：
Lin,CT;Palmer,W;Wu,JY;Chan,L
通讯作者：
Chan,L
培养的鸡胚肝细胞中脂肪生成酶的激素调节。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Back,DW;Wilson,SB;MorrisJr,SM;Goodridge,AG
通讯作者：
Goodridge,AG