MicroRNA expression profile in extracellular vesicles derived from ALV-J infected chicken semen
MicroRNA expression profile in extracellular vesicles derived from ALV-J infected chicken semen
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ALV-J 感染鸡精液细胞外囊泡中的 MicroRNA 表达谱
DOI：
10.1016/j.virusres.2020.198083
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发表时间：
2020-09-01
期刊：
影响因子：
5
通讯作者：
Xie, Qingmei
中科院分区：
文献类型：
--
作者：
Chen, Sheng;Liao, Liqin;Xie, Qingmei
MicroRNA（miRNAs）在许多过程中调控基因表达。细胞外囊泡（EV）中的miRNA也已被广泛研究，而精液EV中的miRNA还没有研究。J亚群禽白血病病毒（ALV-J）可垂直传播，但其传播机制尚不清楚。本研究通过基因本体（GO）和途径富集分析，检测精浆EV中miRNA的表达谱，探讨其与垂直传播的关系。在这里，我们首先通过纳米颗粒跟踪分析、蛋白质印迹和透射电子显微镜实验分离和表征了精液EV。通过对每个EVs miRNA文库进行深度测序，鉴定出9个典型差异表达的miRNA，其中6个上调，3个下调。基因靶点预测、GO注释和KEGG途径富集分析显示了这些miRNA可能的功能。总的来说，这些发现将增加我们对精液EV中miRNA的含量和组成的理解，并为精液EV miRNA调控在ALV-J传播中的重要作用提供新的见解。
MicroRNAs(miRNAs) have been reported to regulate gene expression in many processes. MiRNA in extracellular vesicles (EVs) also have been widely investigated, while there is no studies of miRNAs in seminal EVs. Subgroup J of Avian leukosis virus (ALV-J) can be transmitted vertically, but the mechanism of it is not clear enough. This study was to examine the miRNA expression profile in seminal EVs and inquire into the relation between it and the vertical transmission by performing gene ontology (GO) and pathway enrichment analysis. Here, we first isolated and characterized seminal EVs by Nanoparticle Tracking Analysis,Western Blot and Transmission electron microscopy experiments. By deep sequencing of each EVs miRNA library, 9 typical differentially ex-pressed miRNA, including 6 up-regulated and 3 down-regulated, were identified. Gene target prediction, GO annotation and KEGG pathway enrichment analysis showed possible function associated with these miRNAs. Overall, these findings will increase our understanding of the content and composition of miRNA in seminal EVs and provide new insights into the important role of the seminal EVs miRNAs regulation in ALV-J transmission.