Arachidonic acid stimulates 45calcium efflux and hPL release in isolated trophoblast cells.
Arachidonic acid stimulates 45calcium efflux and hPL release in isolated trophoblast cells.
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花生四烯酸刺激分离的滋养层细胞中的 45 钙流出和 hPL 释放。
DOI：
10.1016/0024-3205(86)90001-9
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发表时间：
1986
期刊：
影响因子：
6.1
通讯作者：
Handwerger,S
中科院分区：
文献类型：
--
作者：
Zeitler,P;Murphy,E;Handwerger,S
该实验室的先前研究表明，花生四烯酸刺激hPL释放的快速、剂量依赖性和可逆性增加，其不依赖于环氧合酶或脂氧合酶代谢。为了进一步研究花生四烯酸刺激hPL释放的机制，在富集的人合体滋养层细胞培养群体中检测了花生四烯酸对45 CaCl预标记的灌流细胞中45 Ca释放的影响。花生四烯酸（10-100 μ M）刺激灌流的胎盘细胞释放45 Ca和hPL的剂量依赖性、快速和可逆的增加。另一方面，即使在高达100 μ M的浓度下，棕榈酸对hPL释放或45 Ca释放的影响也很小。离子载体A23187（1-10 μ M）也刺激hPL释放的剂量依赖性和可逆性增加。由于花生四烯酸增加了细胞钙的动员，如增加的钙流出所反映的，并且由于细胞内钙浓度的增加似乎刺激了hPL释放的增加，这些结果表明花生四烯酸刺激hPL释放可能至少部分是由于脂肪酸对细胞钙动员的影响。
Previous investigations from this laboratory have indicated that arachidonic acid stimulates a rapid, dose-dependent and reversible increase in hPL release which is not dependent on cyclooxygenase or lipoxygenase metabolism. To investigate further the mechanism by which arachidonic acid stimulates the release of hPL, the effect of arachidonic acid on the release of45Ca from perifused cells prelabelled with45CaCl was examined in an enriched cell culture population of term human syncytiotrophoblast. Arachidonic acid (10–100 uM) stimulated a dose-dependent, rapid, and reversible increase in the release of both45Ca and hPL from the perifused placental cells. On the other hand, palmitic acid had little effect on either hPL release or45Ca release even at concentrations as high as 100 uM. Ionophore A23187 (1–10 uM) also stimulated a dose-dependent and reversible increase in hPL release. Since arachidonic acid increases the mobilization of cellular calcium, as reflected by the increased45calcium efflux, and since an increase in the intracellular calcium concentration appears to stimulate an increase in hPL release, these results suggest that the stimulation of hPL release by arachidonic acid may be due, at lease in part, to the effects of the fatty acid on cellular calcium mobilization.