Bacterial endophytes from Lycoris radiata promote the accumulation of Amaryllidaceae alkaloids
Bacterial endophytes from Lycoris radiata promote the accumulation of Amaryllidaceae alkaloids
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石蒜内生菌促进石蒜科生物碱的积累
DOI：
10.1016/j.micres.2020.126501
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发表时间：
2020-10-01
影响因子：
6.7
通讯作者：
Wang, Ren
中科院分区：
文献类型：
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作者：
Liu, Zhilin;Zhou, Jiayu;Wang, Ren
石蒜是石蒜科生物碱的主要来源，具有多种药用活性。然而，这些生物碱在植物中的含量较低，限制了它们的药物开发和利用。在这项研究中，细菌内生菌的能力，以提高五个重要的石蒜科生物碱的积累进行了调查。共分离到188株内生细菌。辐射虫及其组成和多样性。其中14个被证明可以显著增加不同器官中感兴趣的生物碱的浓度，超过对照水平高达11.1倍，对植物生长没有不利影响。另外发现3种内生细菌能显著增加L.对植物中生物碱的浓度没有不利影响，因此植物中生物碱的总产量比对照水平提高了2.4倍。考虑到这些细菌内生菌对植物的促生作用，推测它们分泌的吲哚-3-乙酸和铁载体与固氮能力相结合，可能有助于促进植物生长和增加L.辐射状。据我们所知，本工作首先确定了L.辐射和确定哪些物种促进石蒜科生物碱的积累。本研究为进一步提高L.辐射和扩大我们的药用植物和细菌内生菌之间的相互作用的理解。
Lycoris radiata is the major source of Amaryllidaceae alkaloids, having various medicinal activities. However, the low content of these alkaloids in planta limits their pharmaceutical development and utilization. In this study, the ability of bacterial endophytes to enhance the accumulation of five important Amaryllidaceae alkaloids was investigated. A total of 188 bacterial endophytes were isolated from L. radiata and their composition and diversity were analyzed. Fourteen ones were demonstrated to significantly increase the concentration of the alkaloids of interest in different organs, up to 11.1-fold over the control level, with no adverse influence on the plant growth. An additional 3 bacterial endophytes were found to significantly increase the dry weight of L. radiata with no adverse influence on the concentration of the alkaloids in planta, so the total yield of alkaloids in planta was increased up to 2.4-fold over the control level. Considering the plant growth-promoting abilities of these bacterial endophytes, it is speculated that the indole-3-acetic acid and siderophore secreted by them, combined with their nitrogen fixation ability, may contribute to the enhanced plant growth and the increased alkaloid accumulation in L. radiata. To our knowledge, this work is firstly defining the diversity of culturable bacterial endophytes in L. radiata and determining which species promoted the accumulation of Amaryllidaceae alkaloids. It provides several valuable bacterial inoculants that can be further applied to improve alkaloid production in L. radiata and broadens our understanding of the interactions between a medicinal plant and the bacterial endophytes.