Action of tannin on cellular membranes: Novel insights from concerted studies on lipid bilayers and native cells
Action of tannin on cellular membranes: Novel insights from concerted studies on lipid bilayers and native cells
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DOI：
10.1016/j.bbamem.2019.03.017
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发表时间：
2019-06-01
影响因子：
3.4
通讯作者：
Sivozhelezov, Victor S.
中科院分区：
文献类型：
--
作者：
Borisova, Marina P.;Kataev, Anatoly A.;Sivozhelezov, Victor S.
可水解单宁（3，6-双-O-二没食子酰基-1，2，4-三-O-没食子酰基-β-D-葡萄糖）对细胞膜具有双重作用：（1）它与轮藻细胞的质膜蛋白结合（C-50 = 2.7 +/- 0.3 NM），（2）它在脂质膜中形成离子通道。基于这些事实，单宁与细胞膜的相互作用的分子模型的建议。该模型表明，可水解单宁的分子静电结合到负责Ca 2+依赖性氯电流的膜蛋白的外部基团，并阻止它。一些单宁分子渗透到膜的疏水区域，当达到特定浓度时，它们形成对Cl-选择性的离子传导结构。
Hydrolyzable tannin (3,6-bis-O-digalloyl-1,2,4-tri-O-galloyl-beta-D-glucose) has a dual effect on the cell membrane: (1) it binds to a plasmalemmal protein of the Chara corallina cell (C-50 = 2.7 +/- 0.3 NM) and (2) it forms ionic channels in the lipid membrane. Based on these facts, a molecular model for the interaction of tannins with the cell membrane is proposed. The model suggests that the molecules of hydrolyzable tannin bind electrostatically to the outer groups of the membrane protein responsible for the Ca2+-dependent chloride current and blocks it. Some tannin molecules penetrate into the hydrophobic region of the membrane, and when a particular concentration is reached, they form ion-conducting structures selective toward Cl-.