Evidence that the p75 neurotrophin receptor mediates perineural spread of desmoplastic melanoma
Evidence that the p75 neurotrophin receptor mediates perineural spread of desmoplastic melanoma
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DOI：
10.1016/s0190-9622(96)90728-8
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发表时间：
1996-11-01
影响因子：
13.8
通讯作者：
Bothwell, M
中科院分区：
文献类型：
--
作者：
Iwamoto, S;Odland, PB;Bothwell, M
背景：促结缔组织增生性黑色素瘤的变异体具有明显的嗜神经性倾向。这种嗜神经性可能反映了许旺氏分化。雪旺细胞沿着胚胎神经的迁移受75 kd神经营养因子受体（p75（NTR））及其同源配体之一神经生长因子（NGF）的调节。本研究的目的是验证这一假设，即促纤维增生性黑色素瘤的神经周围扩散机制类似于雪旺细胞的嗜神经性，方法：采用免疫组化法对梭形细胞、促结缔组织增生性黑色素瘤和上皮样黑色素瘤的p75（NTR）进行标记，并与正常对照组进行比较。组织学材料由11例患者的梭形细胞黑素瘤标本组成，其中7例表现出促结缔组织增生性黑素瘤的特征。所有梭形细胞黑色素瘤标本中至少10%的细胞表达p75（NTR），大多数细胞表达p75（NTR）超过50%。结论：p75（NTR）的表达与促结缔组织增生表型密切相关，支持p75（NTR）及其配体参与黑色素瘤嗜神经性的假说。p75（NTR）的免疫标记可能是一个有用的标志物的亲神经表型，以及检测神经周围扩散的组织切片的黑色素瘤。
Background: The desmoplastic variant of melanoma has a striking propensity for neurotropism. This neurotropism may reflect Schwannian differentiation. The migration of Schwann cells along embryonic nerves is reportedly regulated by the 75 kd neurotrophin receptor (p75(NTR)) and one of its cognate Ligands, nerve growth factor (NGF).Objective: The purpose of this study was to test the hypothesis that the mechanism of perineural spread in desmoplastic melanomas is analogous to that of neurotropism in Schwann cells, which apparently depends on expression of p75 neurotrophin receptor and its ligands.Methods: Immunolabeling of p75(NTR) in histologic sections of spindle cell and desmoplastic melanomas was compared and contrasted with that of epithelioid melanomas.Results: The histologic material consisted of spindle cell melanoma specimens from 11 patients, of which seven exhibited features of desmoplastic melanoma. All spindle cell melanoma specimens expressed the p75(NTR) in at least 10% of the cells, and most expressed p75(NTR) in more than 50% of cells. in contrast, 10 of 11 control melanomas of conventional epithelioid phenotype expressed lower levels of p75(NTR) (0% to 10% of cells).Conclusion: There is a strong correlation between expression of p75(NTR) and the desmoplastic phenotype, supporting the hypothesis that the propensity of these melanomas for neurotropism involves p75(NTR) and its ligands. Immunolabeling for p75(NTR) may be a useful marker for the neurotropic phenotype, as well as for detecting perineural spread in histologic sections of melanomas.