A gene that partially complements xeroderma pigmentosum group A cells maps to human chromosome 8.
A gene that partially complements xeroderma pigmentosum group A cells maps to human chromosome 8.
复制标题
部分补充着色性干皮病 A 组细胞的基因映射到人类 8 号染色体。
DOI：
10.1007/bf01235760
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Athwal,RS
中科院分区：
文献类型：
--
作者：
Kaur,GP;Rinaldy,A;Lloyd,RS;Athwal,RS
一个部分补充A组着色性干皮病细胞对紫外线辐射敏感性的基因已被定位在人类8号染色体上。该基因已被分离出来。用代表该基因部分片段的cDNA克隆pEMKR进行定位。根据pEMKR的核苷酸序列，设计了一组寡核苷酸引物，用于从杂交细胞系中扩增DNA。通过聚合酶链式反应和Southern杂交分析，筛选出一组代表人类全基因组的啮齿动物-人类杂交细胞系，对该基因进行染色体定位。只有在含有人类8号染色体的人类和杂交细胞系中才能进行PCR扩增和杂交。因此，pEMKR代表了与已被定位到人类9号染色体上的DNA修复基因XPAC不同的基因。
A gene that partially complements sensitivity of xeroderma pigmentosum cells of group A to UV irradiation has been mapped to human chromosome 8. Isolation of this gene has previously been described. A cDNA clone pEMKR that represents part of this gene was used for mapping. Based upon the nucleotide sequence of pEMKR, a set of oligonucleotide primers were designed for PCR amplification of DNAs from hybrid cell lines. A panel of rodent-human hybrid cell lines representing the total human genome was screened by PCR and Southern blot analysis for chromosomal assignment of this gene. PCR amplification and hybridization occurred only in the case of human and hybrid cell lines that contained human chromosome 8. The pEMKR thus represents a different gene than a DNA repair geneXPACthat has been mapped to human chromosome 9.