ISLET AMYLOID POLYPEPTIDE (IAPP) SECRETION FROM ISLET CELLS AND ITS PLASMA-CONCENTRATION IN PATIENTS WITH NON-INSULIN-DEPENDENT DIABETES-MELLITUS
ISLET AMYLOID POLYPEPTIDE (IAPP) SECRETION FROM ISLET CELLS AND ITS PLASMA-CONCENTRATION IN PATIENTS WITH NON-INSULIN-DEPENDENT DIABETES-MELLITUS
复制标题
DOI：
10.1016/0168-8227(92)90073-z
复制
发表时间：
1992-01-01
影响因子：
5.1
通讯作者：
NANJO, K
中科院分区：
文献类型：
--
作者：
HANABUSA, T;KUBO, K;NANJO, K
胰岛淀粉样多肽（Islet amyloid polypeptide，IAPP/Amylin）是从非胰岛素依赖型糖尿病（NIDDM）患者胰岛淀粉样沉积物中提取的一种新的多肽。然而，其在各种条件下的分泌模式和血浆浓度尚未足够清楚。在这项研究中，我们研究了IAPP分泌胰岛β细胞在体外培养的新生大鼠胰腺和血浆IAPP反应在各种条件下，在体内正常对照组和葡萄糖耐量异常患者的胰岛β细胞。结果表明：（1）在葡萄糖和非葡萄糖刺激下，大鼠胰岛细胞可共同分泌IAPP和胰岛素，（2）正常人空腹血浆IAPP水平为24.9 ± 2.0pg/ml，IAPP/胰岛素摩尔比约为1/7;（3）肥胖患者空腹IAPP水平高，胰岛素依赖型糖尿病患者空腹IAPP水平低，NIDDM患者IAPP/C肽摩尔比低于正常对照组，（4）胰岛素对C肽高反应的肥胖患者在口服葡萄糖负荷时也存在IAPP对C肽的高反应，提示IAPP可能对糖代谢有一定的生理作用。
Islet amyloid polypeptide (IAPP/Amylin) is a novel peptide which was extracted from islet amyloid deposits in patients with non-insulin-dependent diabetes mellitus (NIDDM). However, its pattern of secretions and plasma concentrations under various conditions has not yet been made clear enough. In this study, we examined IAPP secretion from islet beta-cells in vitro using cultured islet cells of neonatal rat pancreas and plasma IAPP responses under various conditions in vivo in normal control subjects and patients with glucose intolerance. Our data revealed that (1) IAPP is co-secreted with insulin from islet cells of the rat pancreas by glucose and non-glucose stimuli; (2) fasting plasma IAPP levels in normal control subjects are 24.9 +/- 2.0 pg/ml and the molar ratio of IAPP/insulin is approximately 1/7; (3) fasting IAPP levels are high in obese patients and low in insulin-dependent diabetic patients, and the molar ratio of IAPP/C-peptide in NIDDM patients is lower than that in normal control subjects, suggesting the basal hyposecretion of IAPP relative to insulin in NIDDM; and (4) the obese patients who had a hyperresponsiveness of insulin relative to C-peptide had the hyperresponsiveness of IAPP relative to C-peptide during an oral glucose load, suggesting that IAPP may have some physiological effect in glucose metabolism.