Direct Protocol for Ambient Mass Spectrometry Imaging on Agar Culture
Direct Protocol for Ambient Mass Spectrometry Imaging on Agar Culture
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DOI：
10.1021/acs.analchem.5b01538
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发表时间：
2015-07-07
影响因子：
7.4
通讯作者：
de Oliveira, Luciana Gonzaga
中科院分区：
文献类型：
--
作者：
Angolini, Celio Fernando F.;Vendramini, Pedro Henrique;de Oliveira, Luciana Gonzaga
在这里，我们描述了一个新的协议，允许直接质谱成像（IMS）的琼脂培养。简单的样品脱水导致薄的固体琼脂，这使得能够直接使用基于喷雾的环境质谱技术。为了证明其适用性，金属清除剂铁载体直接从S. wadayamensis，以及解析和强烈的图像获得使用解吸电喷雾电离（DESI）和容易的环境声喷雾电离（EAST）与明确的选择性空间分布的自由和金属结合的分子，提供线索，他们在细胞代谢中的作用。
Herein we describe a new protocol that allows direct mass spectrometry imaging (IMS) of agar cultures. A simple sample dehydration leads to a thin solid agar, which enables the direct use of spray-based ambient mass spectrometry techniques. To demonstrate its applicability, metal scavengers siderophores were imaged directly from agar culture of S. wadayamensis, and well resolved and intense images were obtained using both desorption electrospray ionization (DESI) and easy ambient sonic-spray ionization (EAST) with well-defined selective spatial distributions for the free and the metal-bound molecules, providing clues for their roles in cellular metabolism.