Changes in arginase isoenzymes pattern in human hepatocellular carcinoma
Changes in arginase isoenzymes pattern in human hepatocellular carcinoma
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DOI：
10.1016/j.bbrc.2008.09.093
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发表时间：
2008-12-12
影响因子：
3.1
通讯作者：
Baranczyk-Kuzma, Anna
中科院分区：
文献类型：
--
作者：
Chrzanowska, Alicja;Krawczyk, Marek;Baranczyk-Kuzma, Anna
肝细胞癌（HCC）是世界范围内最常见的肿瘤之一，好发于肝硬化患者。这些研究是在手术期间从50名HCC患者、40名肝硬化患者和40名对照肝脏获得的组织上进行的。结果发现，肝癌组织中的谷胱甘肽转移酶活性分别比正常肝脏和肝癌组织低近5倍和15倍。免疫印迹法检测肝组织中AI和AII的表达，发现肝细胞癌组织中AI的表达量和AI-mRNA的表达量均低于正常肝组织，而AII的表达量明显高于正常肝组织。由于HCC是精氨酸依赖性的，而精氨酸是细胞生长所必需的，因此AI的降低可能会使这种氨基酸保留在肿瘤细胞内。同时，AII的升高可以增加多胺的水平，多胺是细胞增殖的关键化合物。因此，这两种同工酶似乎都参与了肝癌的发生。(C)2008年爱思唯尔公司All rights reserved.
Hepatocellular carcinoma (HCC) is one of the most common tumors worldwide affecting preferentially patients with liver cirrhosis. The studies were performed on tissues obtained during Surgery from 50 patients with HCC, 40 with liver cirrhosis and 40 control livers. It was found that arginase activity in HCC was nearly 5- and 15-fold lower than in cirrhotic and normal livers, respectively. Isoenzymes AI (so-called liver-type arginase) and AII (extrahepatic arginase) were identified by Western blotting in all Studied tissues, however the amount of AI, as well as the expression of AI-mRNA were lower in HCC, in comparison with normal liver, and those of AII were significantly higher. Since HCC is arginine-dependent, and arginine is essential for cells growth, the decrease of AI may preserve this amino acid within tumor cells. Concurrently, the rise of AII can increase the level of polyamines, compounds crucial for cells proliferation. Thus, both arginase isoenzymes seem to participate in liver cancerogenesis. (C) 2008 Elsevier Inc. All rights reserved.