Crystal structure of plant pectin methylesterase
Crystal structure of plant pectin methylesterase
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DOI：
10.1016/s0014-5793(02)02372-4
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发表时间：
2002-03-13
期刊：
影响因子：
3.5
通讯作者：
Eklund, H
中科院分区：
文献类型：
--
作者：
Johansson, K;El-Ahmad, M;Eklund, H
果胶是植物初生细胞壁的主要成分。在细胞发育过程中，果胶被果胶甲酯酶修饰，赋予细胞壁不同的性质。本文报道了一种植物果胶甲酯酶的晶体结构。的P-螺旋结构体现了一个中央裂缝，内衬几个芳香残基，已被推断为适合果胶结合。活性位点位于裂缝的中心，其中Asp157被认为是亲核试剂，Asp136作为酸/碱，Gln113/Gln135形成阴离子空穴以稳定过渡态。(C)2002年欧洲生物化学学会联合会。由Elsevier Science B.V.出版，版权所有。
Pectin is a principal component in the primary cell wall of plants. During cell development, pectin is modified by pectin methylesterases to give different properties to the cell wall. This report describes the first crystal structure of a plant pectin methylesterase. The P-helical structure embodies a central cleft, lined by several aromatic residues, that has been deduced to be suitable for pectin binding. The active site is found at the center of this cleft where Asp157 is suggested to act as the nucleophile, Asp136 as an acid/base and Gln113/Gln135 to form an anion hole to stabilize the transition state. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.