Identification of differentially expressed circulating exosomal lncRNAs in IgA nephropathy patients
Identification of differentially expressed circulating exosomal lncRNAs in IgA nephropathy patients
复制标题
IgA肾病患者循环外泌体lncRNA差异表达的鉴定
DOI：
10.1186/s12865-020-00344-1
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发表时间：
2020-03-31
期刊：
影响因子：
3
通讯作者：
Yang, Qiongqiong
中科院分区：
文献类型：
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作者：
Guo, Na;Zhou, Qin;Yang, Qiongqiong
BackgroundAlthough immunoglobulin A nephropathy (IgAN) is one of the foremost primary glomerular disease, treatment of IgAN is still in infancy. Non-invasive biomarkers are urgently needed for IgAN diagnosis. We investigate the difference in expression profiles of exosomal long non-coding-RNAs (lncRNAs) in plasma from IgAN patients compared with their healthy first-degree relatives, which may reveal novel non-invasive IgAN biomarkers.MethodsWe isolated exosomes from the plasma of both IgAN patients and their healthy first-degree relatives. High-throughput RNA sequencing and real-time quantitative polymerase chain reaction (qRT-PCR) was used to validate lncRNA expression profiles. Pathway enrichment analysis was used to predict their nearest protein-coding genes.ResultslncRNA-G21551 was significantly down-regulated in IgAN patients. Interestingly, the nearest protein-coding gene of lncRNA-G21551 was found to be encoding the low affinity receptor of the Fc segment of immunoglobulin G (FCGR3B).ConclusionsExosomal lncRNA-G21551, with FCGR3B as the nearest protein-coding gene, was down-regulated in IgAN patients, indicating its potential to serve as a non-invasive biomarker for IgAN.
