TOPOGRAPHY OF EPITOPES ON A POLYMORPHIC SCHIZONT ANTIGEN OF PLASMODIUM-FALCIPARUM DETERMINED BY THE BINDING OF MONOCLONAL-ANTIBODIES IN A 2-SITE RADIOIMMUNOASSAY
TOPOGRAPHY OF EPITOPES ON A POLYMORPHIC SCHIZONT ANTIGEN OF PLASMODIUM-FALCIPARUM DETERMINED BY THE BINDING OF MONOCLONAL-ANTIBODIES IN A 2-SITE RADIOIMMUNOASSAY
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DOI：
10.1111/j.1365-3024.1987.tb00541.x
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发表时间：
1987-11-01
影响因子：
2.2
通讯作者：
MCBRIDE, JS
中科院分区：
文献类型：
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作者：
WILSON, CF;ANAND, R;MCBRIDE, JS
用单克隆抗体双位点放射免疫分析法研究了恶性疟原虫185- 205 K糖蛋白两种不同等位基因型上共同表位和变异表位的分布。这两种分子的构象明显相似。在两种抗原上绘制了两个不同的区域，每个区域包括菌株共同表位和多态性表位。两种PSA的共同表位与三级结构中不同的可变表位密切相关。有人建议，这可能有助于寄生虫逃避宿主的免疫反应。
The topographic distribution of common and variant epitopes on two divergent allelic forms of the 185-205K schizont glycoprotein of Plasmodium falciparum were studied by a two-site radioimmunoassay using monoclonal antibodies. Similarities in the conformation of the two molecules were apparent. On both antigens two distinct regions were mapped, each comprising of both strain-common and polymorphic epitopes. Epitopes common to the two PSAs were found to be closely associated with different variable epitopes in tertiary structure. It is suggested that this may contribute to parasite evasion of the host immune response.