Effects of ionizing radiation and beta-adrenergic stimulation on the expression of early response genes in rat parotid glands.
Effects of ionizing radiation and beta-adrenergic stimulation on the expression of early response genes in rat parotid glands.
复制标题
电离辐射和β-肾上腺素刺激对大鼠腮腺早期反应基因表达的影响。
作者：
P. Mertz;P. Fox;A. Pluta;B. J. Baum;E. Kousvelari
目前对电离辐射对大鼠腮腺基因表达的调控知之甚少。本文研究了体内电离辐射对腮腺功能及早期反应基因c-fos、c-jun和jun B表达的影响，并用异丙肾上腺素刺激β-肾上腺素能受体。用异丙肾上腺素治疗照射的大鼠，腺体重量增加到与未照射的大鼠相似的水平。然而，根据腮腺唾液流率的测量，这种治疗对唾液产量没有影响。单独照射可增加c-fos、c-jun和jun B的表达。照射和异丙肾上腺素联合作用对c-fos和jun B的mRNAs和蛋白水平有额外的影响，尤其是在实验早期(1~8h)。单用异丙肾上腺素可诱导高水平的c-fos和Jun B基因表达，但不能诱导c-Jun基因的表达。而辐射和异丙肾上腺素处理8h后，c-jun基因表达明显增加，提示c-jun基因表达受辐射和异丙肾上腺素联合作用。这些结果提示，原癌基因c-fos、c-jun和jun B的表达可能通过不同的信号转导途径来调节，这些信号转导途径可能被这些外界刺激激活，并可能与电离辐射、电离辐射和异丙肾上腺素引起的大鼠腮腺功能变化有关。
There is little known about the regulation of gene expression in rat parotid glands after exposure to ionizing radiation. The present studies investigate the effects of in vivo ionizing radiation, with subsequent stimulation of beta-adrenergic receptors by isoproterenol, on parotid gland function and on the expression of the early response genes, c-fos, c-jun, and jun B. Ionizing radiation diminished parotid gland weight and saliva output. Treatment of irradiated rats with isoproterenol increased the gland weight to levels similar to those in nonirradiated rats. However, such treatment had no effect on saliva output as indicated by measurements of parotid salivary flow rate. Irradiation alone increased the expression of c-fos, c-jun, and jun B. The combination of irradiation and isoproterenol had an additional effect on the levels of c-fos and jun B mRNAs and proteins particularly at earlier experimental times (1 to 8 h). Isoproterenol alone induced high levels of c-fos and jun B mRNA but not of c-jun mRNA. However, c-jun mRNA was induced markedly by radiation and 8 h of isoproterenol treatment, indicating a combined effect on c-jun gene expression. These observations suggest that the expression of the proto-oncogenes c-fos, c-jun, and jun B is probably regulated through differential signal transduction pathways which may be activated by these external stimuli and may be associated with functional changes induced in the rat parotid gland by ionizing radiation and by ionizing radiation and isoproterenol.
影响因子：
11.2
作者：
L. Witte;Z. Fuks;A. Haimovitz-Friedman;I. Vlodavsky;D. Goodman;A. Eldor
通讯作者：
L. Witte;Z. Fuks;A. Haimovitz-Friedman;I. Vlodavsky;D. Goodman;A. Eldor
DOI：
10.1073/pnas.87.15.5663
发表时间：
1990-08
影响因子：
11.1
作者：
M. Sherman;R. Datta;D. Hallahan;R. Weichselbaum;D. Kufe
通讯作者：
M. Sherman;R. Datta;D. Hallahan;R. Weichselbaum;D. Kufe