ON THE BASICITY OF THE EXCITED STATE OF FLAVINS
ON THE BASICITY OF THE EXCITED STATE OF FLAVINS
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黄素兴奋态的基础
DOI：
10.1111/j.1751-1097.1968.tb08018.x
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发表时间：
1968
影响因子：
3.3
通讯作者：
P. Song
中科院分区：
文献类型：
--
作者：
P. Song
用紫外光（uv）照射胞苷酰（3 ′-5 ′）胞苷（CpC），产生单水合物（C* pC和CpC* 的混合物）和双水合物C* pC*，通过电泳将其分离。这些光产物迅速地转化为CpC并脱氨为U* pC和CpU* 的混合物。在0 ℃下，在pH值为3至8的范围内，评价水合物脱水和脱氨基的速率常数。据观察，C* pC* 的衰减速率常数位于C* pC和CpC* 之间的所有pH值的研究。单水合物和双水合物均在pH 4.5左右显示最小稳定性，在pH 8左右显示最大稳定性。在0 ℃时，C* pC*、C* pC和CpC* 的最大脱水速率常数分别为0.26、0.145和0.35小时-1，最小脱水速率常数分别为0.024、0.011和0.091小时-1。测定了0 ℃时在一定pH值范围内C* pC脱氨为U* pC的速率常数.脱氨基产物的量根据pH值的不同从水合物的约2%至10%不等，在pH值8左右产生最大量。
Cytidylyl (3’-5’) cytidine (CpC) was irradiated with ultraviolet light (uv) to produce the single hydrate (a mixture of C* pC and CpC*) and the double hydrate C* pC* which were separated by electrophoresis. These photoproducts rapidly dehydrate to CpC and deaminate to a mixture of U* pC and CpU*. The rate constants for dehydration and deamination of the hydrates were evaluated for a range of pH values from 3 to 8 at 0 C. It is observed that the rate constants for decay of C* pC* lie between those for C* pC and CpC* for all pH values studied. Both single and double hydrates show minimum stability around pH 4.5 and maximum stability around pH 8. The maximum rate constants for dehydration of C* pC*, C* pC and CpC* are 0.26, 0,145 and 0.35 hr-’respectively and the minimum values are 0.024, 0.011 and 0.091 hr-I respectively all at 0 C. The rate constants for deamination of C* pC to U* pC for a range of pH values at 0 C were measured. The amount of deamination product varies from about 2 to 10 per cent of the hydrate depending on pH with the maximum amount being produced around pH 8.