DECAY-ACCELERATING FACTOR (CD55), A GLYCOSYLPHOSPHATIDYLINOSITOL-ANCHORED COMPLEMENT REGULATORY PROTEIN, IS A RECEPTOR FOR SEVERAL ECHOVIRUSES
DECAY-ACCELERATING FACTOR (CD55), A GLYCOSYLPHOSPHATIDYLINOSITOL-ANCHORED COMPLEMENT REGULATORY PROTEIN, IS A RECEPTOR FOR SEVERAL ECHOVIRUSES
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DOI：
10.1073/pnas.91.13.6245
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发表时间：
1994-06-21
影响因子：
11.1
通讯作者：
FINBERG, RW
中科院分区：
文献类型：
--
作者：
BERGELSON, JM;CHAN, M;FINBERG, RW
埃可病毒是属于小核糖核酸病毒科的人类病原体。衰变加速脂肪（Decay-accelerating fatter，缩写为FATT）是一种糖基磷脂酰肌醇（GPI）锚定的表面蛋白，可保护细胞免受自体补体溶解。抗病毒单克隆抗体可防止埃可病毒7附着在易感细胞上，保护细胞免受感染。当用磷脂酰肌醇特异性磷脂酶C（一种从细胞表面释放GPI锚定蛋白的酶）处理时，HeLa细胞特异性地失去了结合埃可病毒7的能力，这表明病毒受体，如GPI，是一种GPI锚定蛋白。尽管中国仓鼠卵巢细胞不结合埃可病毒7，但表达人ECO 3的转染子有效地结合病毒，并且通过用抗ECO 3单克隆抗体预处理来防止结合。抗病毒抗体可预防至少6种埃可病毒血清型的感染。这些结果表明，Eco是介导几种埃可病毒附着和感染的受体。
Echoviruses are human pathogens belonging to the picornavirus family. Decay-accelerating fatter (DAF) is a glycosylphosphatidylinositol (GPI)-anchored surface protein that protects cells from lysis by autologous complement. Anti-DAF monoclonal antibodies prevented echovirus 7 attachment to susceptible cells and protected cells from infection. HeLa cells specifically lost the capacity to bind echovirus 7 when treated with phosphatidylinositol-specific phospholipase C, an enzyme that releases GPI-anchored proteins from the cell surface, indicating that the virus receptor, like DAF, is a GPI-anchored protein. Although Chinese hamster ovary cells do not bind echovirus 7, transfectants expressing human DAF bound virus efficiently, and binding was prevented by pretreatment with an anti-DAF monoclonal antibody. Anti-DAF antibodies prevented infection by at least six echovirus serotypes. These results indicate that DAF is the receptor mediating attachment and infection by several echoviruses.