MOLECULAR AND BIOLOGICAL CHARACTERIZATION OF A LIGAND FOR CD27 DEFINES A NEW FAMILY OF CYTOKINES WITH HOMOLOGY TO TUMOR-NECROSIS-FACTOR
MOLECULAR AND BIOLOGICAL CHARACTERIZATION OF A LIGAND FOR CD27 DEFINES A NEW FAMILY OF CYTOKINES WITH HOMOLOGY TO TUMOR-NECROSIS-FACTOR
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DOI：
10.1016/0092-8674(93)90133-b
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发表时间：
1993-05-07
期刊：
影响因子：
64.5
通讯作者：
PATRICIABECKMANN, M
中科院分区：
文献类型：
--
作者：
GOODWIN, RG;ALDERSON, MR;PATRICIABECKMANN, M
CD 27是在T和B细胞上发现的表面抗原，其与包括肿瘤坏死因子（TNF）和神经生长因子的受体的分子家族具有同源性。通过直接表达克隆策略，使用由连接至人免疫球蛋白G1分子的恒定结构域的CD27的胞外结构域组成的融合蛋白作为探针，分离编码CD27的配体的cDNA。预测的蛋白质产物是II型跨膜蛋白，其基因定位于19p13，并显示与TNF和CD40配体的同源性。生物学特性表明，克隆的配体诱导共刺激的T细胞的增殖，并促进产生溶细胞性T细胞。
CD27 is a surface antigen found on T and B cells that has homology to a family of molecules including the receptors for tumor necrosis factor (TNF) and nerve growth factor. A cDNA encoding a ligand for CD27 was isolated by a direct-expression cloning strategy using a fusion protein composed of the extracellular domain of CD27 linked to the constant domain of a human immunoglobulin G1 molecule as a probe. The predicted protein product is a type II transmembrane protein whose gene maps to 19p13 and that shows homology to TNF and the ligand for CD40. Biological characterization indicates that the cloned ligand induces the proliferation of costimulated T cells and enhances the generation of cytolytic T cells.