ORIGIN OF HUMAN INFLUENZA-VIRUS SUBTYPES H2N2 AND H3N2
ORIGIN OF HUMAN INFLUENZA-VIRUS SUBTYPES H2N2 AND H3N2
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DOI：
10.1016/0042-6822(78)90153-8
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发表时间：
1978-01-01
期刊：
影响因子：
3.7
通讯作者：
ROTT, R
中科院分区：
文献类型：
--
作者：
SCHOLTISSEK, C;ROHDE, W;ROTT, R
用~（32）P标记人流感病毒新加坡株（H_2N_2）的RNA，经聚丙烯酰胺凝胶电泳分离成8个片段，并与相应的基因功能和/或蛋白质进行了相关性分析。通过分子杂交确定H2 N2病毒的各个基因（片段）与不同甲型流感病毒株的基因（片段）之间的碱基序列同源性。新加坡毒株的片段1、5、7和8与FM 1毒株（H1N1）相比显示出几乎100%的碱基序列同源性，而其它片段之间的同源性明显较低（24-76%）。对于新加坡和香港（H3 N2）毒株，除了编码血凝素（HA，24%）的片段外，所有片段都显示出接近100%的同源性。A型禽流感病毒乌克兰株（Hav 7 Neq 2）HA基因的~（32）P标记片段4与香港株的同源性为92%，而其它至少2个片段的同源性明显较低。H2 N2亚型显然是由H1N1亚型通过重组事件衍生而来的，保留了4个H1N1片段，而另外4个片段是从另一个未知毒株获得的。H3 N2亚型可能来源于H2 N2亚型，保留了H2 N2亚型的7个片段，而编码HA的基因来自鸭乌克兰或另一种高度相关的毒株。
The RNA of the human influenza virus Singapore (H2N2) strain was labeled in vivo by 32P and separated by polyacrylamide gel electrophoresis into 8 segments, which were correlated to the corresponding gene functions and/or proteins. The base sequence homology between the individual genes (segments) of the H2N2 virus and those of different influenza A strain was determined by molecular hybridization. Segments 1, 5, 7 and 8 of the Singapore strain exhibit a base sequence homology of almost 100% as compared to the FM1 strain (H1N1), while the homology between the other segments is significantly lower (24-76%). For the Singapore and Hong Kong (H3N2) strains all segments except that coding for the hemagglutinin (HA, 24%) exhibit a homology close to 100%. The 32P-labeled segment 4 (HA-gene) of the avian influenza A strain duck Ukraine (Hav7Neq2) shows a homology of 92% to Hong Kong, while the homology of at least 2 other segments is significantly lower. The H2N2 subtype is apparently derived from the H1N1 subtype by a recombination event retaining 4 H1N1 segments, whie the other 4 segments are gained from another yet unknown strain. The H3N2 subtype is presumably derived from a H2N2 subtype, retaining 7 segments of the H2N2 subtype, while the gene coding for the HA is obtained from the duck Ukraine or another highly related strain.