Detection of new temperature-dependent conformational transition in lysozyme by carbon-13 nuclear magnetic resonance spectroscopy.
Detection of new temperature-dependent conformational transition in lysozyme by carbon-13 nuclear magnetic resonance spectroscopy.
复制标题
通过碳 13 核磁共振波谱检测溶菌酶中新的温度依赖性构象转变。
DOI：
10.1073/pnas.72.6.2095
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发表时间：
1975
影响因子：
11.1
通讯作者：
P. Jollés
中科院分区：
文献类型：
--
作者：
P. Cozzone;S. Opella;O. Jardetzky;J. Berthou;P. Jollés
A specific temperature-dependent conformational transition of hen egg-white lysozyme, occurring between 20 degree C and 30 degree C in solution, has been detected by 13-C-nuclear magnetic resonance spectroscopy. Selective changes in the chemical shifts of aromatic residues, together with differences in the chemical shifts, and nuclear Overhauser enhancement in the carbonyl, carboxyl, and alpha-carbon regions of the spectrum point to the vicinity of subsites D and E as the primary locus of the structural change.
