High-voltage electron microscopy and conventional transmission electron microscopy of the interface zone between bone and endosteal dental implants.
High-voltage electron microscopy and conventional transmission electron microscopy of the interface zone between bone and endosteal dental implants.
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骨和骨内膜牙种植体之间界面区域的高压电子显微镜和传统透射电子显微镜。
DOI：
10.1002/jbm.820260409
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
McKinney,RV
中科院分区：
文献类型：
--
作者：
Steflik,DE;Sisk,AL;Parr,GR;Hanes,PJ;Lake,F;Song,MJ;Brewer,P;McKinney,RV
用anin模型观察下颌骨与牙髓内种植体之间的界面。采用常规透射电镜和高压透射电镜观察未钙化下颌种植体样品。结果显示了界面支持组织的可变性质。矿化骨通常在距种植体表面50纳米的范围内发现，与该表面仅通过电子致密沉积物分开。骨细胞被包裹在腔隙内的界面附近，通过小管延伸出许多细胞突。在发育中的陷窝的界面处也直接观察到成骨细胞。其他界面区域表现为细纤维状和更多的电子透光形态。此外，其他区域显示由含有胶原原纤维、基质物质和钙化包涵体的细胞外产物的更宽区域组成。由于骨是一种积极生长和重塑的组织，这些不同的形态区域围绕整个种植体区域，似乎证实了组织对牙内种植体的动态反应。此外，HVEM立体学被证明是研究这种组织反应的一个令人兴奋的研究工具。
The interface between mandibular bone and endosteal dental implants was examined with anin vivodog model. Undecalcified mandibular implant samples were observed with both conventional transmission electron microscopy and highvoltage transmission electron microscopy (HVEM). Results demonstrated the variable nature of the interfacial support tissues. Mineralized bone was often found within 50 nm of the implant surface, separated from that surface only by an electron dense deposit. Osteocytes were observed close to the interface encased within lacunae extending numerous cellular processes through canaliculi. An osteoblast was also observed directly at the interface within a developing lacuna. Other interfacial areas exhibited a finely fibrillar and more electron lucent morphology. Furthermore, other areas were shown to be composed of wider zones of extracellular products containing collagen fibrils, ground substance, and calcified inclusions. Because bone is a n actively growing and remodeling tissue, these different morphological zones around the entire area of the implants would appear to confirm the dynamic tissue response to endosteal dental implants. Further, HVEM stereology was shown to be an exciting research tool to investigate this tissue response.