Depiction of metabolome changes in histidine-starved Escherichia coli by CE-TOFMS
Depiction of metabolome changes in histidine-starved Escherichia coli by CE-TOFMS
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DOI：
10.1039/b714176a
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发表时间：
2008-01-01
影响因子：
--
通讯作者：
Soga, Tomoyoshi
中科院分区：
文献类型：
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作者：
Ohashi, Yoshiaki;Hirayama, Akiyoshi;Soga, Tomoyoshi
采用基于毛细管电泳飞行时间质谱（CE-TOFMS）的代谢组学技术，研究了组氨酸营养缺陷型大肠杆菌在组氨酸饥饿条件下的代谢变化。在分析之前，我们准备了一个E。大肠杆菌代谢物组列表的727代谢物在文献中报道。改进了代谢产物提取方法，提高了富磷代谢产物的提取效率。例如，在一个实施例中，ATP和GTP。基于这些结果，同时分析了初级代谢中的375个带电亲水中间体，提供了198种代谢物的定量数据。我们证实，在组氨酸生物合成的中间体的细胞内水平迅速积累，在组氨酸饥饿的条件下，组氨酸水平的下降作出反应。同时，纪律的反应，观察到糖酵解，三羧酸循环，氨基酸和核苷酸的生物合成途径调节氨基酸饥饿。
Metabolic changes in response to histidine starvation were observed in histidine-auxotrophic Escherichia coli using a capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS)-based metabolomics technique. Prior to the analysis, we prepared an E. coli metabolome list of 727 metabolites reported in the literature. An improved method for metabolite extraction was developed, which resulted in higher extraction efficiency in phosphate-rich metabolites, e. g., ATP and GTP. Based on the results, 375 charged, hydrophilic intermediates in primary metabolisms were analysed simultaneously, providing quantitative data of 198 metabolites. We confirmed that the intracellular levels of intermediates in histidine biosynthesis are rapidly accumulated in response to a drop in histidine level under histidine-starved conditions. Simultaneously, disciplined responses were observed in the glycolysis, tricarboxylic acid cycle, and amino acid and nucleotide biosynthesis pathways as regulated by amino acid starvation.