LIM homeobox gene-dependent expression of biogenic amine receptors in restricted regions of the C-elegans nervous system
LIM homeobox gene-dependent expression of biogenic amine receptors in restricted regions of the C-elegans nervous system
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DOI：
10.1016/s0012-1606(03)00447-0
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发表时间：
2003-11-01
影响因子：
2.7
通讯作者：
Hobert, O
中科院分区：
文献类型：
--
作者：
Tsalik, EL;Niacaris, T;Hobert, O
生物胺调节多种行为。它们的功能主要是通过G蛋白偶联的7-跨膜结构域受体（GPCR）介导的，其中16个被预测存在于线虫线虫的基因组序列中。我们在这里描述了这些胺能受体的表达模式，包括两个血清素受体（Ser-1和Ser-4），一个酪胺受体（Ser-2），和两个多巴胺受体（DOP-1和DOP-2）。此外，我们描述了不同的，但部分重叠的Ser-2酪胺受体基因座的不同剪接形式的表达模式。我们发现，每一个胺能受体基因的表达在神经系统的限制性区域，其中许多揭示了显着的重叠与LIM同源框（LHX）基因家族的调控因子的表达。我们证明，在特定的细胞类型中的Lhx基因突变体的几个生物胺受体的表达被废除，从而建立这些Lhx基因在调节神经传递方面的作用。我们扩展这些发现与其他细胞的命运标志物，并表明，lim-4 Lhx基因是必需的几个方面，但不是所有的RID运动神经元分化和lim-6 Lhx基因是必需的RIS interneuron分化的特定方面。我们还使用胺能受体gfp报告基因融合体作为工具来可视化Lhx突变背景中特定神经元的解剖结构，并发现PVD粗糙触摸感觉神经元的精细树突分支模式的发展需要rnec-3 Lhx基因。最后，我们分析了Ser-2酪胺受体的突变等位基因，TTX-3 LHX基因在AIY intereuron类的目标。ser-2突变体没有显示出先前显示的与AIY功能丧失相关的缺陷。(C)2003年爱思唯尔公司All rights reserved.
Biogenic amines regulate a variety of behaviors. Their functions are predominantly mediated through G-protein-coupled 7-transmembrane domain receptors (GPCR), 16 of which are predicted to exist in the genome sequence of the nematode Caenorhabditis elegans. We describe here the expression pattern of several of these aminergic receptors, including two serotonin receptors (ser-1 and ser-4), one tyramine receptor (ser-2), and two dopamine receptors (dop-1 and dop-2). Moreover, we describe distinct but partially overlapping expression patterns of different splice forms of the ser-2 tyramine receptor locus. We find that each of the aminergic receptor genes is expressed in restricted regions of the nervous system and that many of them reveal significant overlap with the expression of regulatory factors of the LIM homeobox (Lhx) gene family. We demonstrate that the expression of several of the biogenic amine receptors is abrogated in specific cell types in Lhx gene mutants, thus establishing a role for these Lhx genes in regulating aspects of neurotransmission. We extend these findings with other cell fate markers and show that the lim-4 Lhx gene is required for several but not all aspects of RID motor neuron differentiation and that the lim-6 Lhx gene is required for specific aspects of RIS interneuron differentiation. We also use aminergic receptor gfp reporter fusions as tools to visualize the anatomy of specific neurons in Lhx mutant backgrounds and find that the development of the elaborate dendritic branching pattern of the PVD harsh touch sensory neuron requires the rnec-3 Lhx gene. Lastly, we analyze a mutant allele of the ser-2 tyramine receptor, a target of the ttx-3 Lhx gene in the AIY intemeuron class. ser-2 mutants display none of the defects previously shown to be associated with loss of AIY function. (C) 2003 Elsevier Inc. All rights reserved.