Developmental expression of chemokine-like factor 1, a novel member of chemokines family, in postnatal rat cerebral cortex
Developmental expression of chemokine-like factor 1, a novel member of chemokines family, in postnatal rat cerebral cortex
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DOI：
10.1016/j.neulet.2012.05.019
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发表时间：
2012-06
影响因子：
2.5
通讯作者：
Zhen-Zhen Wang-Zhen;Yi Zhang;Yu-he Yuan;N. Chen
中科院分区：
文献类型：
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作者：
Zhen-Zhen Wang-Zhen;Yi Zhang;Yu-he Yuan;N. Chen
趋化因子样因子 1 (CKLF1) 与诱导神经母细胞瘤细胞的迁移有关，在胎儿大脑中含量丰富，但在成人大脑中很少。鉴于神经细胞迁移在大脑发育中的重要性，CKLF1 的趋化性可能是大脑发育过程中所必需的。因此，首先了解 CKLF1 在大脑发育过程中的详细表达谱非常重要。然而，CKLF1的发育表达模式仍不清楚。我们的目的是研究 CKLF1 在大鼠出生后大脑皮层发育过程中的时空表达。通过多个时间点的逆转录PCR/免疫印迹，CKLF1的mRNA/蛋白表达在出生时丰富，然后在接下来的两周内逐渐下降，并在成年后几乎消失。免疫组化染色显示，CKLF1在大脑皮层、海马、嗅球、部分特定核团及连合纤维中有明显表达。总之，CKLF1的时间表达模式与出生后发育阶段一致，CKLF1的空间位置是神经细胞迁移的一些目的地或大脑出生后发育过程中通常发生髓鞘形成的区域。
Chemokine-like factor 1 (CKLF1) has been implicated to induce the migration of neuroblastoma cells and is abundant in fetal brain but scarce in adult brain. Given the importance of neural cell migration in brain development, it is possible that the chemotaxis of CKLF1 is required during brain development. Therefore, it is essential to know the detailed expression profiles of CKLF1 during brain development first. However, the developmental expression patterns of CKLF1 still remain unclear. We aimed to investigate the temporal and spatial expressions of CKLF1 during cerebral cortex postnatal development in rats. By reverse-transcription PCR/immunoblotting at multiple time points, the mRNA/protein expressions of CKLF1 were in abundance at birth, then decreased progressively within the next two weeks and almost disappeared in adulthood. By immunohistochemistry staining, an obvious expression of CKLF1 was observed in the cerebral cortex, hippocampus, olfactory bulb, some specific nuclei and commissural fibers. Concluding, the temporal expression pattern of CKLF1 was coincident with the postnatal developmental stages and the spatial locations of CKLF1 were some destinations of neural cell migration or regions where myelination normally occurs during cerebrum postnatal development.