Localization of Y1 receptors for NPY and PYY on vascular smooth muscle cells in rat pancreas.
Localization of Y1 receptors for NPY and PYY on vascular smooth muscle cells in rat pancreas.
复制标题
NPY 和 PYY 的 Y1 受体在大鼠胰腺血管平滑肌细胞上的定位。
DOI：
10.1152/ajpgi.1991.260.2.g250
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Williams,JA
中科院分区：
文献类型：
--
作者：
Sheikh,SP;Roach,E;Fuhlendorff,J;Williams,JA
为了定位肽YY（PYY）在胰腺中的结合位点，我们在用125 I-Tyr 36-PPY孵育的大鼠胰腺的冷冻切片上利用载玻片安装放射自显影技术。饱和放射自显影标记位于胰腺血管，而胰岛，腺泡细胞，导管和神经元素似乎没有被专门标记。采用胶原酶消化法制备大鼠胰腺血管碎片。用125 I-Tyr 36-PYY的结合实验表明，与血管中富集的部分可饱和结合，但不与腺泡结合。非放射性神经肽Y（NPY）和PYY对125 I-Tyr 36-PYY结合的抑制作用相似，半数最大抑制作用为31.2 +/- 5 pM（n = 6）;胰多肽（PP）的效力低10，000倍。由于[Leu 31，Pro34]NPY（一种特异性Y1受体激动剂）抑制与NPY类似的结合，因此将结合位点归类为属于Y1型NPY和/或PYY受体。为了进一步本地化的结合[125 I-Tyr 36]PYY血管内，光和电子显微镜放射自显影制备和定量。放射自显影颗粒主要位于血管平滑肌细胞上，尽管在内皮细胞上也可见饱和定位。它的结论是，在胰腺Y1受体主要位于血管平滑肌细胞。
To localize binding sites for peptide YY (PYY) in the pancreas we utilized a slide-mount autoradiographic technique on frozen sections of rat pancreas incubated with 125I-Tyr36-PPY. Saturable autoradiographic labeling was located over pancreatic blood vessels, whereas islets, acinar cells, ducts, and neural elements did not appear to be specifically labeled. Isolated vascular fragments were prepared by collagenase digestion of rat pancreas. Binding experiments with 125I-Tyr36-PYY showed saturable binding to the fraction enriched in blood vessels but not to acini. Inhibition of 125I-Tyr36-PYY binding by nonradioactive neuropeptide Y (NPY) and PYY were similar, with half-maximal inhibition at 31.2 +/- 5 pM (n = 6); the potency of pancreatic polypeptide (PP) was 10,000 times lower. The binding site was classified as belonging to a Y1 type of NPY and/or PYY receptors, since [Leu31,Pro34]NPY, a specific Y1-receptor agonist, inhibited binding similar to NPY. To further localize the bound [125I-Tyr36]PYY within the blood vessels, light- and electron-microscopic autoradiographs were prepared and quantitated. Autoradiographic grains were located predominantly over vascular smooth muscle cells, although saturable localization was also seen over endothelial cells. It is concluded that in the pancreas Y1 receptors are predominantly located on vascular smooth muscle cells.