Murine response to DNA encoding herpes simplex virus type-1 glycoprotein D targeted to the liver.
Murine response to DNA encoding herpes simplex virus type-1 glycoprotein D targeted to the liver.
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DOI：
10.1016/s0264-410x(99)00438-7
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发表时间：
2000-02
期刊：
影响因子：
5.5
通讯作者：
J. Rogers;B. Hull;P. Fink;H. Chiou;N. J. Bigley
中科院分区：
文献类型：
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作者：
J. Rogers;B. Hull;P. Fink;H. Chiou;N. J. Bigley
将编码单纯疱疹病毒1型糖蛋白D（gD-1）的质粒DNA与缀合至聚-L-赖氨酸的无唾液酸乳清类粘蛋白复合。将其静脉注射入BALB/c小鼠后，该复合物被靶向至肝脏。免疫后第6天免疫化学检测到表达gD-1的肝细胞，而免疫后14天可检测到gD-1 DNA。肝脏中gD-1表达和可检测到的gD-1 DNA的下降与T细胞（主要是CD 4+）的流入相关。ASOR-多聚赖氨酸DNA载体系统促进gD-1的肝表达，并可用于针对单纯疱疹病毒1型的疫苗接种。
Plasmid DNA encoding herpes simplex virus type-1 glycoprotein D (gD-1) was complexed with asialoorosomucoid conjugated to poly-l-lysine. Following its intravenous injection into BALB/c mice, this complex was targeted to the liver. Liver cells expressing gD-1 were detected immunohistochemically through day 6 post-immunization, while gD-1 DNA was detectable through 14 days post-immunization. Decline of gD-1 expression and detectable gD-1 DNA in the liver correlated with influx of T cells, predominantly CD4+. The ASOR-poly-l-lysine DNA carrier system promotes hepatic expression of gD-1 and may be useful in vaccination against herpes simplex virus type-1.