Environmental influences on Actinobacillus actinomycetemcomitans biofilm formation
Environmental influences on Actinobacillus actinomycetemcomitans biofilm formation
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DOI：
10.1016/j.archoralbio.2005.09.002
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发表时间：
2006-04-01
影响因子：
3
通讯作者：
Scannapieco, FA
中科院分区：
文献类型：
--
作者：
Haase, EM;Bonstein, T;Scannapieco, FA
牙周的新鲜临床分离物。病原体伴放线放线杆菌（Actinobacillusactinomycetemcomitans）具有粘附的、粗糙的菌落形态，其在连续的体外传代过程中转化为最小粘附的、光滑的菌落表型。本研究的目的是：（1）比较几株A.（2）研究放线菌在不同环境条件下的生物膜形成动态。使用微量滴定板生物膜测定来评估在具有改变的盐浓度和pH的肉汤中生长的菌株的生物膜形成，并评估预处理膜的效果。扫描电子显微镜（SEM）用于监测形态的微观变化。在通过共聚焦显微镜监测的流动池中测量生物膜形成的动力学。RV通常比SV产生更大的生物膜。然而，在生物膜形成的介质依赖性差异是明显的一些粗糙/光滑对。RV对盐和pH的变化更耐受，对氯己定的耐受性比SV更强。马血清几乎消除，唾液显着减少，生物膜形成的SV相反的RV。扫描电镜观察表明，随着环境的变化，其形貌没有发生变化。在流动池中，RV产生由小接触面积锚定的小菌落塔，而SV产生高度降低的开放式结构。在流通池中7天后，可以证明粗糙到平滑的表型转变。综上所述，菌种、生长介质和调理膜均影响生物膜的形成。RV产生具有独特结构的生物膜，其可用于保护细菌免受环境扰动。(c)2005爱思唯尔有限公司保留所有权利。
Fresh clinical isolates of the periodontal. pathogen Actinobacillus actinomycetemcomitans have an adherent, rough colony morphology that transforms into a minimally adherent, smooth colony phenotype during successive in vitro passage. The objectives of this study were: (1) to compare biofilm formation of the rough (RVs) and smooth variants (SVs) of several strains of A. actinomycetemcomitons grown under various environmental conditions and (2) to examine the dynamics of biofilm formation. A microtitre plate biofilm assay was used to evaluate biofilm formation of strains grown in broth with modified salt concentration and pH, and to evaluate the effect of pre-conditioning films. Scanning electron microscopy (SEM) was used to monitor microscopic changes in morphology. Dynamics of biofilm formation were measured in a flowcell monitored by confocal microscopy. The RVs generally produced greater biofilm than the SVs. However, medium-dependent differences in biofilm formation were evident for some rough/smooth pairs. The RVs were more tolerant to changes in salt and pH, and more resistant to chlorhexidine than the SVs. Horse serum virtually eliminated, and saliva significantly reduced, biofilm formation by the SVs in contrast to the RVs. SEM revealed no alteration in morphology with change of environment. In a flowcell, the RVs produced towers of microcolonies anchored by a small contact area, whereas the SVs produced an open architecture of reduced height. After 7 days in a flowcell, the rough to smooth phenotype transition could be demonstrated. In conclusion, strain, growth medium and conditioning film all affect biofilm formation. The RVs produce biofilms of unique architecture that may serve to protect the bacterium from environmental perturbations. (c) 2005 Elsevier Ltd. All rights reserved.